Molecular Cloning of Rat USF2 cDNA and Characterization of Splicing Variants

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概要

• 論文の詳細を見る

• The complete nucleotide sequence of rat USF2 cDNA was determined. In addition to the full length clone (USF2FL), four isoforms (⊿1,⊿2,⊿3,and ⊿4) suggested to be generated by alternative splicing were isolated. USF2⊿1 and ⊿2 lacked 27 and 67 internal amino acid residues, respectively. USF2⊿3 and ⊿4 lacked most of the entire sequence but encoded short peptides of an N-terminal portion of USF2FL. Overexpression of USF2FL increased the transcription of the human high affinity IgE receptor (FcεRI) α chain gene through specific binding to the CAGCTG motif in the first intron. On the other hand, overexpression of USF2⊿1 or ⊿2 reduced the transcription of the human FcεRI α chain gene. Both USF2FL and USF2⊿1 bound to CACGTG as well as CAGCTG, while USF2⊿2 bound to CACGTG but not to CAGCTG. These results suggested the presence of a different and definitive role of each variant in the expression of the α chain gene.

• 社団法人日本農芸化学会の論文
• 2001-01-23

著者

• 奥村 克純

  三重大院・生資・分子細胞生物学

• OKUMURA KO

  Department of Urology, School of Medicine, Jutendo University

• Ra C

  Atopy (allergy) Research Center Juntendo University School Of Medicine

• Takahashi K

  School Of Life Sciences Tokyo Univ. Of Pharmacy And Life Sciences

• Yokota T

  Foods & Pharmaceuticals Research & Development Laboratory Asahi Breweries Ltd.

• RA Chisei

  Department of Molecular Cell Immunology and Allergology, Advanced Medical Research Center, School of

• Takahashi Kayoko

  Central Research Lnstitute Meiji Milk Products Co. Ltd.

• Nishiyama Chiharu

  Department Of Agricultural Chemistry The University Of Tokyo

• Nishiyama Makoto

  Biotechnology Research Center The University Of Tokyo

• Yokota Toyokazu

  Foods & Pharmaceuticals Research & Development Laboratory Asahi Breweries Ltd.

• Ra Chisei

  Allergy And Atopy Research Center Juntendo University School Of Medicine

• Ra Chisei

  Department Of Immunology Juntendo University School Of Medicine

• TAKAHASHI Kyoko

  Foods & Pharmaceuticals Research & Development Laboratory, Asahi Breweries, Ltd.

• OHTAKE Yasuyuki

  Foods & Pharmaceuticals Research & Development Laboratory, Asahi Breweries, Ltd.

• NISHIYAMA Chiharu

  Foods and Pharmaceuticals Research and Development Laboratory, Asahi Breweries Ltd.

• Ohtake Y

  Foods & Pharmaceuticals Research & Development Laboratory Asahi Breweries Ltd.

• Ohtake Yasuyuki

  Foods And Pharmaceuticals Research And Development Laboratory Asahi Breweries Ltd.

• Okumura Ko

  Atopy (allergy) Research Center Juntendo University School Of Medicine

• Okumura Ko

  Department Of Engineering Physics Chubu University

• Yokota T

  Faculty Of Agriculture Shizuoka University

• Yuuki T

  Asahi Breweries Ltd. Ibaraki Jpn

• Takahashi Kyoko

  Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'

• Kageyama T

  Kyoto Univ. Aichi Jpn

• Yokota Takao

  Iss Science Project Office Institute Of Space And Astronautical Science Japan Aerospace Exploration

• Okumura Ko

  Department Of Dermatology Juntendo University School Of Medicine

• Takahashi Kenji

  Laboratory Of Molecular Biochemistry School Of Life Science Tokyo University Of Pharmacy And Life Sc

• Nishiyama Makoto

  Atopy (Allergy) Research Center, Juntendo University School of Medicine

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