Direct Expression of the Extracellular Portion of Human FcεRIα Chain as Inclusion Bodies in Escherichia coli
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概要
- 論文の詳細を見る
The extracellular portion of the α chain of the human high-affinity IgE receptor (FcεRIα) was expressed as inclusion bodies in Escherichia coli. In immunoblot analysis, two bands were reactive to human IgE and mouse anti-human FcεRIα monoclonal antibodies. N-terminal sequencing showed that the two bands were equivalent to the soluble FcεRIα with a methionine residue at the N-terminus (Met-1-172) and 23-172,in which the N-terminal 22 residues of the soluble FcεRIα have been removed, possibly by degradation in E. coli cells. IgE-binding to CHO cells expressing FcεRI was inhibited by the addition of the recombinant products prepared by the refolding procedure from inclusion bodies. The system for the expression of soluble human FcεRIα in E. coli presented in this study and its further improvement would be useful for the production of the protein as a potent therapeutic and for analysis of the IgE-FcεRIα interaction.
- 社団法人日本農芸化学会の論文
- 2001-01-23
著者
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奥村 克純
三重大院・生資・分子細胞生物学
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OKUMURA KO
Department of Urology, School of Medicine, Jutendo University
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Ra C
Atopy (allergy) Research Center Juntendo University School Of Medicine
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RA Chisei
Department of Molecular Cell Immunology and Allergology, Advanced Medical Research Center, School of
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Tokura Tomoko
Atopy (allergy) Research Center Juntendo University School Of Medicine
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Takai T
Atopy (allergy) Research Center Juntendo University School Of Medicine
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Ra Chisei
Allergy And Atopy Research Center Juntendo University School Of Medicine
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Ra Chisei
Department Of Immunology Juntendo University School Of Medicine
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TAKAI Toshiro
Department of Immunology Allergy Research Center, Juntendo University School of Medicine
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Okumura Ko
Atopy (allergy) Research Center Juntendo University School Of Medicine
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Okumura Ko
Department Of Engineering Physics Chubu University
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Takai Toshiro
Atopy (allergy) Research Center
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Okumura Ko
Department Of Dermatology Juntendo University School Of Medicine
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