Effects of a 2-step Culture with Cytokine Combinations on Megakaryocytopoiesis and Thrombopoiesis from Carbon-ion Beam-irradiated Human Hematopoietic Stem/progenitor Cells

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概要

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• To evaluate whether the continuous treatment of two cytokine combinations is effective in megakary-ocytopoiesis and thrombopoiesis in hematopoietic stem/progenitor cells exposed to heavy ion beams, the effects of a 2-step culture by a combination of recombinant human interleukin-3 (IL-3) + stem cell factor (SCF) + thrombopoietin (TPO), which just slightly protected against carbon-ion beam-induced damages, and a combination of IL-3 + TPO, which selectively stimulated the differentiation of the hematopoietic stem/progenitor cells to megakaryocytes and platelets, were examined. CD34^+-hematopoietic stem/progen-itor cells isolated from the human placental and umbilical cord blood were exposed to carbon-ion beams (LET = 50 keV/μm) at 2 Gy. These cells were cultured under three cytokine conditions. The number of megakaryocytes, platelets and hematopoietic progenitors were assessed using a flow cytometer and a clonogenic assay at 14 and 21 days after irradiation, respectively. However, the efficacy of each 2-step culture was equal or lower than that of using the IL-3 + SCF + TPO combination alone and the 2-step culture could not induce megakaryocytes and platelets from hematopoietic stem/progenitor cells exposed to high LET-radiation such as carbon-ion beams. Therefore, additional cytokines and/or hematopoietic promoting compounds might be required to overcome damage to hematopoietic cells by high LET radiation.

• 2008-07-16

著者

• TAKAHASHI Kenji

  Department of Radiological Life Sciences, Hirosaki University Graduate School of Health Sciences

• KASHIWAKURA Ikuo

  Department of Radiological Life Sciences, Hirosaki University Graduate School of Health Sciences

• Yoshino Hironori

  Department of Radiological Life Sciences, Division of Medical Life Sciences, Hirosaki University Gra

• Monzen Satoru

  Department of Radiological Life Sciences, Division of Medical Life Sciences, Hirosaki University Gra

• Abe Yoshinao

  Lab. Of Radiology Hirosaki Univ. Graduate School Of Medicine

• KASAI Kiyomi

  Natl. Inst. Rad. Sci.

• Eguchi-kasai Kiyomi

  Res. Center For Radiation Protection National Inst. Of Radiological Sciences

• Eguchi-kasai Kiyomi

  Radiation Effect Mechanisms Research Group Research Center For Radiation Protection National Institu

• Takahashi Kenji

  Dep. Of Radiological Life Sciences Hirosaki Univ. Graduate School Of Health Sciences

• Takahashi K

  School Of Life Sciences Tokyo Univ. Of Pharmacy And Life Sciences

• Takahashi Kayoko

  Central Research Lnstitute Meiji Milk Products Co. Ltd.

• ABE Yoshinao

  Sch. Med. Hirosaki Univ.

• Takahashi Kenji

  Department Of Orthopaedics Graduate School Of Medical Science Kyoto Prefectural University Of Medici

• Takahashi K

  Tokyo Univ. Agriculture And Technol. Tokyo Jpn

• ABE Yoshinao

  Laboratory of Radiology, Hirosaki University School of Medicine

• Yoshino Hironori

  Department Of Physics And Electronics Faculty Of Engineering Osaka Prefecture University

• Kashiwakura Ikuo

  Department Of Radiological Life Sciences Division Of Medical Life Sciences Hirosaki University

• Takahashi Ken-ichi

  Department Of Industrial Chemistry Faculty Of Engineering Chiba Institute Of Technology

• Kashiwakura Ikuo

  Dep. Of Radiological Life Sciences Hirosaki Univ. Graduate School Of Health Sciences

• Takahashi Kyoko

  Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'

• Kageyama T

  Kyoto Univ. Aichi Jpn

• Eguchi-kasai Kiyomi

  Radiation Effect Mechanisms Res. Group Res. Center For Radiation Protection National Inst. Of Radiol

• Takahashi Kenji

  School Of Life Sciences Tokyo University Of Pharmacy And Life Sciences

• Takahashi Kenji

  Department Of Biophysics And Biochemistry Graduate School Of Science The University Of Tokyo

• Abe Yoshinao

  Laboratory Of Radiology Hirosaki University Graduate School Of Medicine

• Kashiwakura Ikuo

  Graduate School Of Health Sciences Hirosaki University

• Takahashi Kenji

  Department Of Atomic Science And Nuclear Engineering Faculty Of Engineering Hokkaido University

• Takahashi Kenji

  Laboratory Of Molecular Biochemistry School Of Life Science Tokyo University Of Pharmacy And Life Sc

• Kashiwakura Ikuo

  北海道薬科大学 放射薬品

• Takahashi Kenji

  Department Of Applied Biological Chemistry Graduate School Of Agriculture Kinki University

• Yoshino Hironori

  Department of Radiological Life Sciences, Hirosaki University Graduate School of Health Sciences

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