Inhibitory Effects of Isoliquiritigenin and Licorice Extract on Voltage-Dependent K^+ Currents in H9c2 Cells
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概要
- 論文の詳細を見る
The effect of isoliquiritigenin (ISL), a component of licorice, on the voltage-dependent, ultra-rapidly activating delayed-rectifier K+ current (IKur) was examined in H9c2 cells, a cell-line derived from rat cardiac myoblasts. IKur was recorded using the whole-cell patch clamp method with a pipette solution containing 140 mM K+. Depolarizing voltage pulses of 200-ms duration were given with 10-mV steps every 10 s from −40 mV holding potential. ISL inhibited IKur in a concentration-dependent manner. The median inhibitory concentration (IC50) of ISL was approximately 0.11 μM and the Hill coefficient was 0.71. Using CHO cells expressing Kv1.5 IKur channels, ISL also inhibited Kv1.5 IKur, but less potently than the IKur current in H9c2 cells. Furthermore, in H9c2 cells, the licorice extract itself inhibited IKur in a manner similar to ISL. We conclude that ISL, one component of licorice, is a potent inhibitor of K+ channels, which specifically in H9c2 cells could be Kv2.1, and that this inhibition may be involved in various pharmacological effects of licorice.
- 社団法人 日本薬理学会の論文
- 2008-12-20
著者
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TAKAHASHI Kenji
Department of Radiological Life Sciences, Hirosaki University Graduate School of Health Sciences
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Kimura Junko
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Toho University
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MURAKAWA Masahiro
Department of Anesthesiology, Fukushima Medical University School of Medicine
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Maeda Ryo
Department Of Pharmacology
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Sakamoto Kazuho
Department of Pharmacology, School of Medicine, Fukushima Medical University, Japan
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Ono Tomoyuki
Department of Pharmacology, School of Medicine, Fukushima Medical University, Japan
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Yang Jing
Department Of Oral Health Promotion Institute For Oral Science Matsumoto Dental University
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Kimura J
Dep. Of Pharmacology School Of Medicine Fukushima Medical Univ. Jpn
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Maeda Ryo
Department Of Biophysics Graduate School Of Science Kyoto University
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Takahashi Kenji
Department Of Orthopaedics Graduate School Of Medical Science Kyoto Prefectural University Of Medici
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NOGUCHI Chisato
Department of Anesthesiology, Fukushima Medical University, School of Medicine
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TAKASUGI Hiroshi
Department of Pharmacology
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Kimura J
Department Of Pharmacology Fukushima Medical University School Of Medicine
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Yang Jing
中華人民共和国
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Ono Tomoyuki
Department Of Pharmacology Fukushima Medical University School Of Medicine
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Ono Tomoyuki
Department Of Pharmacology Fukushima Medical College
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Yang Jing
Department Of Cardiology Renmin Hospital Of Wuhan University Wuhan Cardiovascular Research Institute
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TAKAHASHI Kenji
Japan Pharmaceutical Manufacturers Association
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Noguchi Chisato
Department Of Anesthesiology Fukushima Medical University School Of Medicine
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Sakamoto Kazuho
Department Of Molecular And Cellular Pharmacology Graduate School Of Pharmaceutical Sciences Nagoya
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Murakawa Masahiro
Department Of Anesthesiology Fukushima Medical University School Of Medicine
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Murakawa Masahiro
Department Of Anesthesia Kyoto University Hospital
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Takahashi Kenji
Department Of Biophysics And Biochemistry Graduate School Of Science The University Of Tokyo
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Sakamoto Kazuho
Department Of Pharmacology School Of Medicine Fukushima Medical University
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Takahashi Kenji
Department Of Pharmacology
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Takahashi Kenji
Department Of Atomic Science And Nuclear Engineering Faculty Of Engineering Hokkaido University
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Takahashi Kenji
Department Of Applied Biological Chemistry Graduate School Of Agriculture Kinki University
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Yang Jing
Department of Biology and Biochemistry, University of Bath
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