Inhibitory Effect of Thiopental on Ultra-rapid Delayed Rectifier K^+ Current in H9c2 Cells
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概要
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Using the whole-cell voltage clamp technique, we investigated the effects of thiopental on membrane currents in H9c2 cells, a cell line derived from embryonic rat heart. Thiopental blocked a rapidly activating, very slowly-inactivating ultra-rapid type IKur-like outward K+ current in a concentration-dependent manner. The half-maximal concentration (IC50) of thiopental was 97 μM with a Hill coefficient of 1.2. The thiopental-sensitive current was also blocked by high concentrations of nifedipine (IC50 = 9.1 μM) and 100 μM chromanol 293B, a blocker of slowly activating delayed rectifier K+ current (IKs), but was insensitive to E-4031, an inhibitor of rapidly activating delayed rectifier K+ current (IKr). TEA (tetraethylammonium) at 5 mM and 4-AP (4-aminopiridine) at 1 mM reduced the K+ current to 30.8 ± 12.2% and 20.5 ± 6.5% of the control, respectively. Using RT-PCR, we detected mRNAs of Kv2.1, Kv3.4, Kv4.1, and Kv4.3 in H9c2 cells. Among those, Kv2.1 and Kv3.4 have IKur-type kinetics and are therefore candidates for thiopental-sensitive K+ channels in H9c2 cells. This is the first report showing that thiopental inhibits IKur. This effect of thiopental may be involved in its reported prolongation of cardiac action potentials.
- 社団法人 日本薬理学会の論文
- 2005-10-20
著者
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Kimura Junko
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Toho University
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Suzuki Hitoshi
Department of Cardiology and Hematology, Fukushima Medical University
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Ono Tomoyuki
Department of Pharmacology, School of Medicine, Fukushima Medical University, Japan
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Kimura J
Dep. Of Pharmacology School Of Medicine Fukushima Medical Univ. Jpn
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Momoi Nobuo
Department Of Pediatrics Fukushima Medical University School Of Medicine
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SUZUKI Hideki
Department of Medical Information, Gunma Prefectural College of Health Sciences
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Kimura J
Department Of Pharmacology Fukushima Medical University School Of Medicine
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Ono Tomoyuki
Department Of Pharmacology Fukushima Medical University School Of Medicine
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Ono Tomoyuki
Department Of Pharmacology Fukushima Medical College
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MATSUOKA Isao
Department of Pharmacology, Fukushima Medical University, School of Medicine
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Matsuoka Isao
Department Of Pharmacology Fukushima Medical College
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Maeda Sachiko
Department Of Pharmacology Fukushima Medical University School Of Medicine
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Maeda Sachiko
Department Of Biology Faculty Of Science Kobe University
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SHIKAMA Yayoi
Department of Pharmacology, Fukushima Medical University, School of Medicine
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Shikama Yayoi
Department Of Pharmacology Fukushima Medical University School Of Medicine
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Suzuki Hideki
Department Of Pediatrics Fukushima Medical University School Of Medicine
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Matsuoka Isao
Department Of Anesthesiology Ilikai Medical Inc Kakizoe Hospital
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Suzuki Hitoshi
Department Of Animal Science Faculty Of Agriculture Tohoku University
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SUZUKI Hideki
Department of Agricultural Chemistry, The University of Tokyo
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Momoi Nobuo
Department of Pediatrics, Fukushima Medical University, School of Medicine
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MOMOI NOBUO
Department of Pediatrics
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