The Effects of X-Irradiation on Ex Vivo Expansion of Cryopreserved Human Hematopoietic Stem/Progenitor Cells
スポンサーリンク
概要
- 論文の詳細を見る
In our previous study (Life Sciences 84: 598, 2009), we demonstrated that placental/umbilical cord blood-derived mesenchymal stem cell-like stromal cells have the effect to support the regeneration of freshly prepared X-irradiated hematopoietic stem/progenitor cells (HSPCs). Generally, HSPCs are supplied from companies, institutions, and cell banks that cryopreserve them for clinical and experimental use. In this study, the influence of cryopreservation on the responses of HSPCs to irradiation and co-culture with stromal cells is assessed. After cryopreservation with the optimal procedure, 2 Gy-irradiated HSPCs were cultured with or without stromal cells supplemented with combination of interleukin-3, stem cell factor, and thrombopoietin. The population of relatively immature CD34+/CD38- cells in cryopreserved cells was significantly higher than in fresh cells prior to cryopreservation; furthermore, the hematopoietic progenitor populations of CD34+/CD45RA+ cells and CD34+/CD117+ cells in cryopreserved cells were significantly lower than that in fresh cells. However, the rate of expansion in the cryopreserved HSPCs was lower than in the fresh HSPCs. In the culture of cryopreserved cells irradiated with 2 Gy, the growth rates of CD34+ cells, CD34+/CD38- cells, and hematopoietic progenitors were greater than growth rates of their counterparts in the culture of fresh cells. Surprisingly, the effect to support the hematopoiesis in co-culture with stromal cells was never observed in the X-irradiated HSPCs after cryopreservation. The present results demonstrated that cryopreserving process increased the rate of immature and radio-resistant HSPCs but decreased the effects to support the hematopoiesis by stromal cells, thus suggesting that cryopreservation changes the character of HSPCs.
- 2010-03-16
著者
-
HAYASHI Naoki
Department of Radiological Life Sciences, Hirosaki University Graduate School of Health Sciences
-
TAKAHASHI Kenji
Department of Radiological Life Sciences, Hirosaki University Graduate School of Health Sciences
-
KASHIWAKURA Ikuo
Department of Radiological Life Sciences, Hirosaki University Graduate School of Health Sciences
-
Takahashi Kenji
Department Of Orthopaedics Graduate School Of Medical Science Kyoto Prefectural University Of Medici
-
Kashiwakura Ikuo
Department Of Radiological Life Sciences Division Of Medical Life Sciences Hirosaki University
-
Hayashi Naoki
Department Of Radiological Life Sciences Hirosaki University Graduate School Of Health Sciences
-
Hayashi Naoki
Department Of Applied Chemistry Waseda University
-
Hayashi Naoki
Department Of Anatomy Shiga University Of Medical Science
-
Takahashi Kenji
Department Of Biophysics And Biochemistry Graduate School Of Science The University Of Tokyo
-
Takahashi Kenji
Department Of Applied Biological Chemistry Graduate School Of Agriculture Kinki University
関連論文
- The Effects of X-Irradiation on Ex Vivo Expansion of Cryopreserved Human Hematopoietic Stem/Progenitor Cells
- Proteoglycans Regulate the Chemotaxis of Dendritic Cells Derived from Human Peripheral Blood Monocytes
- Effects of Proteoglycan Extracted from Nasal Cartilage of Salmon Heads on Maturation of Dendritic Cells Derived from Human Peripheral Blood Monocytes
- Effects of a 2-step Culture with Cytokine Combinations on Megakaryocytopoiesis and Thrombopoiesis from Carbon-ion Beam-irradiated Human Hematopoietic Stem/progenitor Cells
- Inductive Potential of Recombinant Human Granulocyte Colony-Stimulating Factor to Mature Neutrophils from X-Irradiated Human Peripheral Blood Hematopoietic Progenitor Cells
- Biological Activity of Some Cyanobiphenyl Derivatives
- Liquid Crystallinity and Biological Activity of a Novel Amphiphilic Compound
- Maternal and Neonatal Factors Associated with the High Yield of Mononuclear Low-Density/CD34^+ Cells from Placental/Umbilical Cord Blood
- Transplantation of Mesenchymal Stem Cells to Prevent Radiation-induced Intestinal Injury in Mice
- Comparison of anti-rheumatic effects of local RNAi-based therapy in collagen induced arthritis rats using various cytokine genes as molecular targets
- Enhanced expression of interleukin-6, matrix metalloproteinase-13, and receptor activator of NF-κB ligand in cells derived from osteoarthritic subchondral bone
- Cationic polymer-mediated genetic transduction into cultured human chondrosarcoma-derived HCS-2/8 cells
- Dopamine Release in the Ventromedial Hypothalamus of Growing Chickens Decreases when They are Fed a Lysine Devoid Diet
- Maximum intensity projection as a tool to diagnose early rheumatoid arthritis
- Age-related changes in the hemodynamics of the femoral head as evaluated by early phase of bone scintigraphy
- Evaluation of the hemodynamics of the femoral head compared with the ilium, femoral neck and femoral intertrochanteric region in healthy adults : measurement with positron emission tomography (PET)
- Molecular Cloning of a cDNA for Proctase B from Aspergillus niger var. macrosporus and Sequence Comparison with Other Aspergillopepsins I
- Bi_2Sr_2Ca_1Cu_2O_X Thin Film Deposition by Q-switched YAG Laser
- Purification and Further Characterization of Enteropeptidase from Porcine Duodenum
- Purification and Characterization of an Extracellular Metalloprotease from Pseudomonas fluorescens^1
- Occurrence of a Novel 350-kDa Serine Proteinase in the Fluid of Porcine Ovarian Follicles and Its Increase during Their Maturation(Reproductive Biology)
- Preparation and Structural Analysis of Micro-patterned Pb(Zr,Ti)O_3 Film by Metalorganic Chemical Vapor Deposition
- Metalorganic Chemical Vapor Deposition of Epitaxial Perovskite SrIrO_3 Films on (100)SrTiO_3 Substrates
- Thermal Stability of SrRuO_3 Bottom Electrode and Electric Property of Pb(Zr, Ti)O_3 Thin Film Deposited on SrRuO_3
- Combination analysis of three polymorphisms for predicting the risk for steroid-induced osteonecrosis of the femoral head
- ApoB C7623T polymorphism predicts risk for steroid-induced osteonecrosis of the femoral head after renal transplantation
- Genetic analysis of steroid-induced osteonecrosis of the femoral head
- Determining the morphological features of breast cancer and predicting the effects of neoadjuvant chemotherapy via diagnostic breast imaging
- The In Vitro Epithelial Repair Capacity in Acid-Injured Alveolar Cell Model
- Totally Organic Polymer-Based Electrochromic Cell Using TEMPO-Substituted Polynorbornene as a Counter Electrode-Active Material
- Change of cross-linked telopeptide of type I collagen (ICTP) and other bone resorption markers in patients with bone fragility fractures
- Late Pulmonary Metastasis of a Rectal Cancer Resected 20 Years after Low Anterior Resection
- Ileal Shwannoma in which Blood Loss Scintigraphy was Useful for Diagnosis
- Individual Differences in the Radiosensitivity of Hematopoietic Progenitor Cells Detected in Steady-State Human Peripheral Blood
- Slipped capital femoral epiphysis first occurring in early childhood with subsequent development on the other side in later childhood
- Absence of osteonecrosis of the femoral head following bilateral femoral neck fracture with a high degree of displacement
- IIA-20 The therapeutic effects of basic fibroblast growth factor controlled release system on experimental osteoarthritis in the rabbit knee(THE 46TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY)
- Time-sequential changes in biomechanical and morphological properties of articular cartilage in cryopreserved osteochondral allografting
- Inhibitory Effects of Isoliquiritigenin and Licorice Extract on Voltage-Dependent K^+ Currents in H9c2 Cells
- A proposed catalytic mechanism of aspergilloglutamic peptidase from Aspergillus niger
- Effects of Replacement of Lys25 with Gln on the Conformation of Ribonuclease T_1: Sequence-Specific ^1H NMR Resonance Assignments of Gln25 Ribonuclease T_1 by Two-Dimensional NMR Spectroscopy
- Effect of Strain in Epitaxially Grown SrRuO_3 Thin Films on Crystal Structure and Electric Properties
- Mucinous carcinoma of Vater's ampulla with a unique extension along the main pancreatic duct
- Reliability and validity of the Japanese Orthopaedic Association hip score
- Genetic association of a polymorphism of the cAMP-responsive element binding protein-binding protein with steroid-induced osteonecrosis after kidney transplantation
- Enlerged Frequency Upshift from DC to AC Radiation Converter using Boundary Effect of Plasma-filled Waveguide
- The Influence of Gender- and Age-related Differences in the Radiosensitivity of Hematopoietic Progenitor Cells Detected in Steady-state Human Peripheral Blood
- The three-dimensional structure of aspergilloglutamic peptidase from Aspergillus niger
- Leader Peptidase from Escherichia coli: Overexpression, Characterization, and Inactivation by Modification of Tryptophan Residues 300 and 310 with N-Bromosuccinimide
- Morphological study of the endotoxin translocation in the ileal epithelium of monkey
- Characterization of Hafnium Oxide Thin Films by Source Gas Pulse Introduced Metalorganic Chemical Vapor Deposition Using Amino-Family Hf Precursors
- Growth Behavior of c-Axis-Oriented Epitaxial SrBi_2Ta_2O_9 Films on SrTiO_3 Substrates with Atomic Scale Step Structure
- Hydrostatic Pressure Induces Cytokine Production in Human Periodontal Ligament Cells
- Purification and Characterization of Turtle Pepsinogen and Pepsin
- Osteoblasts derived from osteophytes produce interleukin-6, interleukin-8, and matrix metalloproteinase-13 in osteoarthritis
- Heavy Ion Beam Irradiation Regulates the mRNA Expression in Megakaryocytopoiesis from Human Hematopoietic Stem/Progenitor Cells
- Differential Induction from X-irradiated Human Peripheral Blood Monocytes to Dendritic Cells
- Influence of extracellular matrix on the expression of inflammatory cytokines, proteases, and apoptosis-related genes induced by hydrostatic pressure in three-dimensionally cultured chondrocytes
- Asporin and transforming growth factor-β gene expression in osteoblasts from subchondral bone and osteophytes in osteoarthritis
- Structural Evidence That Scytalidolisin (Formerly Scytalidopepsin A) Is a Serine-Carboxyl Peptidase of the Sedolisin Family
- Gene therapies for joint diseases
- Minimum 10-year radiographic follow-up of a cementless acetabular component for primary total hip arthroplasty with a bulk autograft
- Purification and Characterization of a Major Collagenase from Streptomyces parvulus
- Effects of Local and Whole Body Irradiation on the Appearance of Osteoblasts During Wound Healing in Tooth Extraction Sockets in Rats
- LOCAL MASS TRANSFER EROM A SINGLE CYLINDER AND TUBE BANKS VIBRATING SINUSOIDALLY IN A FLUID AT REST
- The Promoting Activity on Human Megakaryocytopoiesis and Thrombopoiesis by Liquid Crystal-Related Compounds
- Occupation and social experience : Factors influencing attitude towards people with schizophrenia
- Schizophrenic outpatient perceptions of psychiatric treatment and psychotic symptomatology : An investigation using structural equation modeling
- Nitroxide Radicals for Highly Efficient Redox Mediation in Dye-sensitized Solar Cells
- Combined Impact of the Association between Prepregnancy Body Mass Index and Gestational Weight Gain on the Placental/Umbilical Cord Blood Volume Collected
- Left Thoracotomy Approach in Reoperative Off-Pump Coronary Revascularization : Bypass Grafting from the Left Axillary Artery or Descending Thoracic Aorta
- Purification and Characterization of a Major Collagenase from Streptomyces parvulus
- The Influence of Gender- and Age-related Differences in the Radiosensitivity of Hematopoietic Progenitor Cells Detected in Steady-state Human Peripheral Blood
- Activation of C-Kit by Stem Cell Factor Induces Radioresistance to Apoptosis through ERK-dependent Expression of Survivin in HL60 Cells
- Effects of group musical therapy on inpatients with chronic psychoses : A controlled study
- A VIRTUAL MASS AND A DRAG COEFFICIENTS FOR AN OSCILLATING PORTICLE
- Substituent effects on the glass transition phenomena of polyorganosilsesquioxane particles prepared by two-step acid–base catalyzed sol–gel process
- Ascorbic Acid Enhances Radiation-induced Apoptosis in an HL60 Human Leukemia Cell Line
- Infraoptic Course of the Anterior Cerebral Artery Originating From the Extradural Internal Carotid Artery Associated With Contralateral Internal Carotid Artery Agenesis and Multiple Intracerebral Aneurysms : Case Report
- Structural Evidence That Scytalidolisin (Formerly Scytalidopepsin A) Is a Serine-Carboxyl Peptidase of the Sedolisin Family
- Parental perceptions of borderline personality disorders in video-recorded interviews
- A Case of Esophageal Squamous Cell Carcinoma Metastatic to the Breast
- Preparation and Characterization of Polyaminophenylsilsesquioxane Particles by Two-step Acid-Base-catalyzed Sol-Gel Process
- Preparation of needle-like α-Fe2O3 particles and influences of their morphology on the electrochemical behavior in all-solid-state lithium batteries
- Kinematic Measurement of Knee Prosthesis from Single-Plane Projection Images(Bioengineering)
- Recent trends in orthopedic surgery performed in Japan for rheumatoid arthritis
- Phenomenological features of auditory hallucinations and their symptomatological relevance
- Involvement of Intracellular Reactive Oxygen Species and Mitochondria in the Radiosensitivity of Human Hematopoietic Stem Cells
- Chromosome 17 polysomy in circulating tumor cells in patients with metastatic breast cancer : a case series
- Degradation of interleukin 8 by the serine protease MucD of Pseudomonas aeruginosa
- Prognostic value of HER2-positive circulating tumor cells in patients with metastatic breast cancer
- Serum HER2 levels determined by two methods in patients with metastatic breast cancer
- Effects of Ionizing Radiation on Proliferation and Differentiation of Mouse Induced Pluripotent Stem Cells
- Complementation of the exoS gene in the pvdE pyoverdine synthesis gene-deficient mutant of Pseudomonas aeruginosa results in recovery of the pvdE gene-mediated penetration through the intestinal epithelial cell barrier but not the pvdE-mediated virulence
- Ultrasonography- and/or mammography-guided breast conserving surgery for ductal carcinoma in situ of the breast : experience with 87 lesions
- Novel Acridine-Based N-Acyl-homoserine Lactone Analogs Induce Endoreduplication in the Human Oral Squamous Carcinoma Cell Line SAS
- Transplantation of Mesenchymal Stem Cells to Prevent Radiation-induced Intestinal Injury in Mice
- Correlations between the detection of stress-associated hormone/oxidati ve stress markers in umbilical cord blood and the physical condition of the mother and neonate
- Characteristics of reactive oxygen metabolites in serum of early teenagers in Japan
- Influence of obstetric factors on the yield of mononuclear cells, CD34^+ cell count and volume of placental/umbilical cord blood