The three-dimensional structure of aspergilloglutamic peptidase from Aspergillus niger

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概要

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• Aspergilloglutamic peptidase from Aspergillus niger is a novel pepstatin-insensitive acid endopeptidase distinct from the well-studied aspartic peptidases, and thus is an interesting target for protein structure/function studies. In the present study, we have determined the three-dimensional structure of the enzyme by X-ray crystallography to a 1.4-Å resolution. The results revealed that the enzyme has a unique structure, composed of two seven-stranded anti-parallel β-sheets which form a β-sandwich structure and appear to have a partial two-fold symmetry, suggesting its possible evolution by gene duplication and that the glutamic acid-110 and glutamine-24 in the heavy chain form a catalytic dyad, consistent with our results obtained by site-directed mutagenesis. (Communicated by Masanori OTSUKA, M.J.A.)

• 日本学士院の論文

著者

• TAKAHASHI Kenji

  Department of Radiological Life Sciences, Hirosaki University Graduate School of Health Sciences

• Sasaki Hiroshi

  Department of Biological Technology, Faculty of Science and Engineering,Senshu University of Ishinom

• Nakagawa Atsushi

  Institute for Protein Research, Osaka Univ.

• KOJIMA Masaki

  School of Life Sciences, Tokyo University of Pharmacy and Life Sciences

• Muramatsu T

  Nagoya Univ. Graduate School Of Medicine Nagoya Jpn

• KUBOTA Keiko

  School of Life Sciences, Tokyo University of Pharmacy and Life Sciences

• Takahashi Kenji

  Department Of Orthopaedics Graduate School Of Medical Science Kyoto Prefectural University Of Medici

• Tanokura M

  Univ. Tokyo Tokyo Jpn

• Tanokura Masaru

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences Univers

• Tanokura Masaru

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Sawano Yoriko

  Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U

• SUGANUMA Megumi

  Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U

• MURAMATSU Tomonari

  Biophysics Division, National Cancer Center Research Institute

• Sawano Yoriko

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Miyakawa Takuya

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences Univers

• Kojima Masaki

  School Of Life Science Tokyo University Of Pharmacy And Life Science

• Kubota Keiko

  School Of Life Sciences Tokyo University Of Pharmacy And Life Sciences

• Nakagawa Atsushi

  Institute For Protein Research Osaka University

• Takahashi Kenji

  Department Of Biophysics And Biochemistry Graduate School Of Science The University Of Tokyo

• Sasaki Hiroshi

  Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University

• Takahashi Kenji

  Department Of Atomic Science And Nuclear Engineering Faculty Of Engineering Hokkaido University

• Takahashi Kenji

  Department Of Applied Biological Chemistry Graduate School Of Agriculture Kinki University

• TANOKURA Masaru

  Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo

• Nakagawa Atsushi

  Institute for Protein Research, Osaka University

• KUBOTA Keiko

  School of Life Science, Tokyo University of Pharmacy and Life Science

• TAKAHASHI Kenji

  Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo

• SASAKI Hiroshi

  Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo

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