Heavy Ion Beam Irradiation Regulates the mRNA Expression in Megakaryocytopoiesis from Human Hematopoietic Stem/Progenitor Cells

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概要

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• Heavy ion beams are a high-LET radiation that has greater biological effect than electron beams or X-rays. However, little is known about the effect of heavy ion beams on the proliferation and differentiation of human hematopoietic stem/progenitor cells (HSPCs). The present study examined the effect of heavy ion beams on gene expression in human HSPCs, especially during early stage of megakaryocytopoiesis. Human CD34+ cells were exposed to monoenergetic carbon-ion beams (290 MeV/nucleon, LET = 50 KeV/m) that were generated by an accelerator (Heavy Ion Medical Accelerator in Chiba). The expression of various genes related to early hematopoiesis, megakaryocytopoiesis/erythropoiesis, cytokine receptors and oxidative stress were analyzed by real-time RT-PCR. Friend leukemia virus integration 1, an early hematopoiesis-related gene, showed significantly higher mRNA expression than the control at 6 hr after irradiation. In contrast, no significant differences were observed in almost all of the other early hematopoiesis-related genes, cytokine receptor-coded genes and megakaryocytopoiesis/erythropoiesis-differentiation pathway-related genes, respectively. An analysis of the response of the genes to oxidative stress revealed the expression of heme oxygenase 1 to show a 1.5-fold and 11.9-fold increase from the day 0 control at 24 hr after 0.5 Gy and 2 Gy irradiation, respectively. Similarly, the NAD(P)H dehydrogenase-quinone 1 expression also showed a 22.0-fold and a 21.8-fold increase at 6 hr in comparison to the initial control. These results showed that the heavy ion beams affect megakaryocytopoiesis/ erythropoiesis differentiation of human HSPCs on the gene expression level.

• Journal of Radiation Research 編集委員会の論文
• 2009-09-16

著者

• TAKAHASHI Kenji

  Department of Radiological Life Sciences, Hirosaki University Graduate School of Health Sciences

• KASHIWAKURA Ikuo

  Department of Radiological Life Sciences, Hirosaki University Graduate School of Health Sciences

• Yoshino Hironori

  Department of Radiological Life Sciences, Division of Medical Life Sciences, Hirosaki University Gra

• Monzen Satoru

  Department of Radiological Life Sciences, Division of Medical Life Sciences, Hirosaki University Gra

• Itoh Ken

  Department of Stress Response Science, Hirosaki University School of Medicine

• KASAI Kiyomi

  Natl. Inst. Rad. Sci.

• Eguchi-kasai Kiyomi

  Res. Center For Radiation Protection National Inst. Of Radiological Sciences

• Eguchi-kasai Kiyomi

  Radiation Effect Mechanisms Research Group Research Center For Radiation Protection National Institu

• Takahashi Kenji

  Dep. Of Radiological Life Sciences Hirosaki Univ. Graduate School Of Health Sciences

• Takahashi K

  School Of Life Sciences Tokyo Univ. Of Pharmacy And Life Sciences

• Takahashi Kayoko

  Central Research Lnstitute Meiji Milk Products Co. Ltd.

• MARUYAMA Atsushi

  Department of Environmental Solution Technology, Ryukoku University

• ABE Yoshinao

  Sch. Med. Hirosaki Univ.

• Takahashi Kenji

  Department Of Orthopaedics Graduate School Of Medical Science Kyoto Prefectural University Of Medici

• Takahashi K

  Tokyo Univ. Agriculture And Technol. Tokyo Jpn

• ABE Yoshinao

  Laboratory of Radiology, Hirosaki University School of Medicine

• Yoshino Hironori

  Department Of Physics And Electronics Faculty Of Engineering Osaka Prefecture University

• Kashiwakura Ikuo

  Department Of Radiological Life Sciences Division Of Medical Life Sciences Hirosaki University

• Takahashi Ken-ichi

  Department Of Industrial Chemistry Faculty Of Engineering Chiba Institute Of Technology

• Kashiwakura Ikuo

  Dep. Of Radiological Life Sciences Hirosaki Univ. Graduate School Of Health Sciences

• KASAI EGUCHI

  Radiation Effect Mechanisms Research Group, Research Center for Radiation Protection, National Insti

• Takahashi Kyoko

  Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'

• Kageyama T

  Kyoto Univ. Aichi Jpn

• Maruyama Atsushi

  Hirosaki Univ. Graduate School Of Medicine Hirosaki

• Maruyama Atsushi

  Department Of Biomolecular Engineering Tokyo Institute Of Technology

• Maruyama Atsushi

  Department Of Anesthesia Kasukabe-chuo General Hospital

• Takahashi Kenji

  School Of Life Sciences Tokyo University Of Pharmacy And Life Sciences

• Takahashi Kenji

  Department Of Biophysics And Biochemistry Graduate School Of Science The University Of Tokyo

• Abe Yoshinao

  Laboratory Of Radiology Hirosaki University Graduate School Of Medicine

• Kashiwakura Ikuo

  Graduate School Of Health Sciences Hirosaki University

• Takahashi Kenji

  Department Of Atomic Science And Nuclear Engineering Faculty Of Engineering Hokkaido University

• Takahashi Kenji

  Laboratory Of Molecular Biochemistry School Of Life Science Tokyo University Of Pharmacy And Life Sc

• Itoh Ken

  Department Of Stress Response Science Hirosaki University Graduate School Of Medicine

• Takahashi Kenji

  Department Of Applied Biological Chemistry Graduate School Of Agriculture Kinki University

• Yoshino Hironori

  Department of Radiological Life Sciences, Hirosaki University Graduate School of Health Sciences

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