The Effects of (-)-Epigallocatechin-3-Gallate on the Proliferation and Differentiation of Human Megakaryocytic Progenitor Cells

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概要

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• Epigallocatechin-3-gallate (EGCg) has been widely recognized as a powerful antioxidant and free radical scavenger. The effects of EGCg on the proliferation and differentiation of X-irradiated megakaryocytic progenitor cells (colony-forming unit-megakaryocyte, CFU-Meg) using CD34^+ cells prepared from human placental and umbilical cord blood have been shown. In the absence of exogenous thrombopoietin (TPO), no colonies are observed in cultures containing or lacking EGCg (1nM-100μM). In the presence of TPO, in contrast, EGCg significantly promotes CFU-Meg-derived colony formations within the 10-100nM range. A 1.5-fold increase in the total number of CFU-Meg has been counted compared with the control. These favorable effects of EGCg are also observed in the culture of CD34^+ cells before and after X irradiation with 2 Gy. Moreover, in order to investigate the function of EGCg promoting megakaryocytopoiesis and thrombopoiesis in ex vivo cultures, both non-irradiated and X-irradiated CD34^+ cells are grown in liquid cultures supplemented with TPO. In both cultures, EGCg increases the total number of cells and megakaryocytes. It has been suggested that the favorable effects of EGCg reduce the risk factor from radiation damage in megakaryocytopoiesis.

• 日本放射線影響学会の論文
• 2006-06-30

著者

• Miura Tomisato

  弘前大学 理工学研究科物質理工学

• Abe Yoshinao

  Lab. Of Radiology Hirosaki Univ. Graduate School Of Medicine

• Takahashi Kenji

  Dep. Of Radiological Life Sciences Hirosaki Univ. Graduate School Of Health Sciences

• Takahashi K

  School Of Life Sciences Tokyo Univ. Of Pharmacy And Life Sciences

• Takahashi Kayoko

  Central Research Lnstitute Meiji Milk Products Co. Ltd.

• ABE Yoshinao

  Sch. Med. Hirosaki Univ.

• Mori Tetsuya

  Department Of Radiology And Nuclear Medicine Hyogo Brain And Heart Center

• KUWABARA MIKINORI

  Laboratory of Radiation Biology, Graduate School of Veterinary Medicine

• Takahashi K

  Tokyo Univ. Agriculture And Technol. Tokyo Jpn

• ABE Yoshinao

  Laboratory of Radiology, Hirosaki University School of Medicine

• INANAMI Osamu

  Laboratory of Radiation Biology, Department of Environmental Veterinary Sciences, Graduate School of

• MONZEN Satoru

  Laboratory of Radiological Technology, Hirosaki University School of Health Sciences

• MORI Takao

  Kitanihonkagaku

• TAKAHASHI Kenji

  Laboratory of Radiological Technology, Hirosaki University School of Health Sciences

• KASHIWAKURA Ikuo

  Laboratory of Radiological Technology, Hirosaki University School of Health Sciences

• Kuwabara Mikinori

  弘前大学 保健学研究科放射線生命科学

• Kuwabara Mikinori

  Laboratory Of Radiation Biology Graduate School Of Veterinary Medicine

• Kuwabara Mikinori

  Laboratory Of Radiation Biology Department Of Environmental Veterinary Sciences Graduate School Of V

• Inanami Osamu

  弘前大学 保健学研究科放射線生命科学

• Inanami Osamu

  Laboratory Of Radiation Biology Department Of Environmental Veterinary Medical Sciences Graduate Sch

• Inanami Osamu

  Laboratory Of Radiation Biology Graduate School Of Veterinary Medicine Hokkaido University

• KUWABARA Mikinori

  Labs. Radiat. Biol.

• Kuwabara M

  Hokkaido Univ. Sapporo Jpn

• Mori T

  First Institute Of New Drug Research Otsuka Pharmaceutical Co. Ltd.

• Takahashi Ken-ichi

  Department Of Industrial Chemistry Faculty Of Engineering Chiba Institute Of Technology

• Inanami Osamu

  Lab. Radiat. Biol. Grad. Sch. Vet. Med. Hokkaido Univ.

• Kashiwakura Ikuo

  Dep. Of Radiological Life Sciences Hirosaki Univ. Graduate School Of Health Sciences

• Kuwabara Mikinori

  Lab. Radiat. Biol. Grad. Sch. Vet. Med. Hokkaido Univ.

• Takahashi Kyoko

  Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'

• Kageyama T

  Kyoto Univ. Aichi Jpn

• Takahashi Kenji

  School Of Life Sciences Tokyo University Of Pharmacy And Life Sciences

• Abe Yoshinao

  Laboratory Of Radiology Hirosaki University Graduate School Of Medicine

• Kashiwakura Ikuo

  Graduate School Of Health Sciences Hirosaki University

• Takahashi Kenji

  Laboratory Of Molecular Biochemistry School Of Life Science Tokyo University Of Pharmacy And Life Sc

• Kashiwakura Ikuo

  北海道薬科大学 放射薬品

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