Improved Physical and Biochemical Features of a Collagen Membrane by Conjugating with Soluble Egg Shell Membrane Protein
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概要
- 論文の詳細を見る
Soluble egg shell membrane protein (SEP) prepared from the egg shell membrane by performic acid oxidation and pepsin digestion was applied to prepare a membrane conjugated with pepsin-solubilized collagen (PSC) by cross-linking with water-soluble carbodiimide. SEP enabled a conjugated membrane to be constructed with a well-developed network structure having thicker collagen fibrils and higher stability than the PSC membrane alone, as indicated by reduced solubility and collagenase-digestion, increased denaturation temperature, and superior flexibility. The SEP-PSC membrane (SEP:PSC of 1:1, w/w) had lower sweatiness than the PSC membrane. Fibroblasts and keratinocytes adhered to the SEP-PSC-coated wells (SEP:PSC of 1:10, w/w) as a fundamental model of an artificial skin similar to that to the PSC-coated wells due to similar expression level of several cell-adhesion proteins. An SEP-PSC coating accelerated the cell growth of fibroblasts, whereas the cell growth of keratinocytes did not change, or decreased slightly due to more rapid differentiation indicated by a more substantial expression of differentiation marker proteins than a PSC-coating without SEP.
- 社団法人 日本農芸化学会の論文
- 2006-04-23
著者
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TAKAHASHI Koji
Department of Pediatrics, Nihon Kokan Hospital
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YOSHIDA Tadashi
Department of Pathophysiology, Faculty of Pharmaceutical Sciences, Hoshi University
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Hattori Shunji
Nippi Research Institute Of Biomatrix
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Hattori Shunji
Nippi Research Institute Of Biomatrix Senju-midoricho Nippi Inc.
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Takahashi K
School Of Life Sciences Tokyo Univ. Of Pharmacy And Life Sciences
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HATTORI Makoto
Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture an
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Yoshida Tadashi
Dep. Of Applied Biological Sci. Tokyo Univ. Of Agriculture And Technol.
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INO Tamao
Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture an
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YOSHIMURA Keiji
Tokyo Metropolitan Leather Technology Center
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Ino Tamao
Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And
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Hattori M
Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And
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Hattori Makoto
Dep. Of Applied Biological Sci. Tokyo Univ. Of Agriculture And Technol.
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Takahashi K
Dep. Of Applied Biological Sci. Fac. Of Agriculture Tokyo Univ. Of Agriculture And Technol.
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Hattori Makoto
Department Of Applied Biological Science Tokyo University Of Agriculture And Technology
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Yoshida Tadashi
Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And
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Takahashi Koji
Department Of Chemistry And Chemical Engineering Yamagata University
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Hattori Makoto
Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And
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Hattori Makoto
Department Of Agricultural Chemistry The University Of Tokyo
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Takahashi Koji
Department Of Aeronautics And Astronautics Kyushu University
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Hattori Shunji
Nippi Res. Inst. Of Biomatrix Jpn
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Yoshida Tadashi
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University:(present Office)frontie
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Takahashi Koji
Institute Of Symbiotic Science And Technology Division Of Agriscience And Bioscience Tokyo University Of Agriculture And Technology
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Hattori Makoto
Department of Applied Biological Science, Tokyo University of Agriculture and Technology
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TAKAHASHI Koji
Departent of Materials Science and Engineering,Faculty of Engineering,Yamagata University
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