KERATINOCYTE APOPTOSIS ON TYPE I COLLAGEN GEL CAUSED BY A LACK OF LAMININ 5 AND 10/11 DEPOSITION

概要

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Culturcd human foreskin kcratinocytes (HFKs) adhcrc to and grow on non-fibrous collagcn via integrin α2β1. During incubation, the receptors used for adhesion are changed to integrins α3β1, α6β4 and those receptors bound to laminin 5 which is deposited by keratinocyte themselves. In this report, we examined the behaviors of HFKs and transformed keratinocytes on collagen fibril gels. These cells adhered to and spread on collagen gels using integrin α2β1. After several hours on the collagen gels, however, cells became round and apoptosis occurred. The behaviors of keratinocytes contrasted to that of fibroblast that grew well even on the collagen gel. At the point of apoptosis, integrins α2β1 and α3β1 were not found in the contact region of the HFKs. Also, deposition of laminin 5 on collagen gels was not found despite the detection of the laminin 5 and laminin 10/11 by RT-PCR, and soluble laminin 5 protein by Western blotting. These results indicate that the collagen gel has different effects than non-fibrous collagen on HFKs and that the interactions of integrin α3β1 and laminin 5/10/11 are indispensable for maintenance of keratinocyte adhesion and survival.
日本結合組織学会の論文