Epigallocatechin-3-O-gallate Inhibits Fibroblast Contraction of Floating Collagen Gel : Interaction between Epigallocatechin-3-O-gallate and Platelet Derived Growth Factor

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概要

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• The effects of (−)-epigallocatechin gallate (EGCG) on the contraction of floating collagen gel by fibroblasts were investigated. EGCG inhibited collagen gel contraction dose-dependently. On the basis of the fact that platelet-derived growth factor (PDGF) is one of the serum components with stimulatory activity in collagen gel contraction, we examined the possibility that interaction between EGCG and PDGF may be involved in this inhibition mechanism. We confirmed this by recombinant PDGF-BB in the present system and we found that EGCG inhibited PDGF-stimulated collagen gel contraction. The results of affinity chromatography indicated that PDGF was bound by EGCG immobilized on agarose gel as detected by enzyme-linked immunoassay and Western blotting. These findings suggest that binding of EGCG to PDGF is at least partly involved in the mechanism of inhibition of collagen gel contraction by EGCG.

• 2004-08-23

著者

• Hattori Shunji

  Nippi Research Institute Of Biomatrix

• ISEMURA Mamoru

  School of Food and Nutritional Sciences and Center of Excellence for the 21st Century, University of

• Isemura Mamoru

  School Of Food And Nutritional Sciences And Center Of Excellence For The 21^<st> Century Unive

• SUZUKI Yasuo

  School of Food and Nutritional Sciences, and Center of Excellence for the 21^ Century, Universit

• Hattori Shunji

  Nippi Res. Inst. Of Biomatrix Jpn

• Suzuki Yasuo

  School Of Food And Nutritional Sciences And Center Of Excellence For The 21^<st> Century Unive

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