Isolation of a 41-kDa Protein with Cell Adhesion Activity for Animal Tumor Cells from the Mushroom Hypsizigus marmoreus by Affinity Chromatography with Type IV Collagen Immobilized on Agarose

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概要

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• A type IV collagen-binding protein of 41 kDa was isolated from the mushroom Hypsizigus marmoreus and the protein was designated as HM41. The Western blotting analysis with anti-HM41 antibodies demonstrated that HM41 was unrelated to HM23,which had been shown to have an affinity for type IV collagen. The microsequence analysis of the membrane-blotted peptides generated by fragmentation with cyanogen bromide showed no homologous proteins reported. HM41 had cell adhesion-promoting activity for murine Lewis lung carcinoma LL2 cells and human fibrosarcoma HT1080 cells. These results indicate that HM41 is a hitherto undescribed fungus protein that can interact both with animal extracellular matrix protein type IV collagen and with animal tumor cells.

• 社団法人日本農芸化学会の論文
• 2000-04-23

著者

• Aoyagi Y

  Niigata Univ. School Of Medicine Niigata Jpn

• Aoyagi Yutaka

  Department Of Third Internal Medicine Niigata University School Of Medicine

• Aoyagi Yutaka

  Department Of Radiology Tokyo Dental College

• Aoyagi Yutaka

  Tokyo University Of Pharmacy And Life Sciences School Of Pharmacy

• ISEMURA Mamoru

  School of Food and Nutritional Sciences and Center of Excellence for the 21st Century, University of

• Munakata Hiroshi

  Department Of 2nd Biochemistry Kinki University School Of Medicine

• MUTO Haruhiko

  Shizuoka Prefecture Forestry and Forest Products Research Institute

• ISEMURA Satoko

  Nippon Dental University Junior College at Niigata

• SHOJI Yutaka

  School of Food and Nutritional Sciences, University of Shizuoka

• Shoji Y

  Kyushu Univ. Fukuoka Jpn

• Isemura S

  Nippon Dental University Junior College At Niigata

• Isemura Satoko

  Nippon Dental University Junior Collage At Niigata

• Aoyagi Y

  Tokyo University Of Pharmacy And Life Sciences School Of Pharmacy

• 伊勢村 護

  Cellular Biochemistry School Of Food And Nutritional Sciences University Of Shizuoka

• Isemura Mamoru

  Laboratory Of Cellular Biochemistry Graduate School Of Nutritional And Environmental Sciences Univer

• Aoyagi Yutaka

  Department Of Gastroenterology And Hepatology Niigata University Graduate School Of Medical And Dent

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