Separation of Phospholipase A_2 in Habu Snake Venom by Glycyrrhizin (GL)-Affinity Column Chromatography and Identification of a GL-Sensitive Enzyme

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概要

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• By means of glycyrrhizin (GL)-affinity and Mono S column chromatographies (HPLC), at least four GL-binding proteins (p25,p17,p15-1 and p15-2) in the two Superdex fractions (P-II and P-III fractions) from Habu snake venom were selectively purified. By determination of their N-terminal partial amino acid sequences, a metalloprotease (p25) and three GL-binding phospholipases A_2 (gbPLA_2s) [PA2Y (p17), PA21 (p15-1) and PA2B (p15-2)] were identified. PA2B (lysine-49 PLA_2) was found to be the most sensitive to GL because (i) it strongly bound to a GL-affinity column; and (ii) its enzyme activity was selectively inhibited by low dose (ID_<50>=approx. 1.5μM) of GL, but not by GA. Furthermore, these three gbPLA_2s were phosphorylated by casein kinase II (CK-II) in vitro and GL inhibited the CK-II-mediated stimulation of their enzyme activities in vitro.

• 公益社団法人日本薬学会の論文
• 1998-06-15

著者

• OHTSUKI Kenzo

  Laboratory of Genetical Biochemistry and Signal Biology, Graduate School of Medical Sciences, Kitasa

• Munakata Hiroshi

  Department of Urology and Biochemistry, Kinki University, School of Medicine

• Ohtsuki Kenzo

  Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato

• ABE Yoshinori

  Laboratory of Genetical Biochemistry, Kitasato University School of Allied Health Sciences

• SHIMOYAMA Yoshihito

  Laboratory of Genetical Biochemistry, Kitasato University School of Allied Health Sciences

• FURUYA Teisuke

  Laboratory of Genetical Biochemistry, Kitasato University School of Allied Health Sciences

• 大久保 研之

  Department Of Clinical Chemistry And Laboratory Medicine Faculty Of Medicine Kyushu University

• MUNAKATA Haruka

  Hokkaido College of Pharmacy

• TAKASAKI Chikahisa

  Department of Biomolecular Sciences, Graduate School of Life Science, Tohoku University

• Munakata Hiroshi

  Department Of 2nd Biochemistry Kinki University School Of Medicine

• Munakata Hiroshi

  Dept. Of Biochemistry Kinki Univ. School Of Med.

• MUTO Haruhiko

  Shizuoka Prefecture Forestry and Forest Products Research Institute

• Abe Yoshinori

  Laboratory Of Genetical Biochemistry Kitasato University School Of Allied Health Sciences

• Furuya Teisuke

  Laboratory Of Genetical Biochemistry Kitasato University School Of Allied Health Sciences

• Munakata Hidekazu

  Department Of Biochemistry Kinki University School Of Medicine

• Shimoyama Y

  Kitasato Univ. Sagamihara Jpn

• Yanahira Shuichi

  Technology And Research Institute Snow Brand Milk Products Co. Ltd.

• Takasaki Chikahisa

  Department Of Biomolecular Science Graduate School Of Life Sciences Tohoku University

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