Examination of growth promotion factors for smooth muscle cells of hypertrophied bladders in rats following partial outlet obstruction

概要

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It was reported that outlet obstruction increased the bladder weight in comparison with the controls from one week after the obstruction. This increase continued until 20 weeks after the operation ; however, there was no increase from 30 weeks post operation. Pathologically, thickening of the wall (mainly the muscle layer) was seen. Previously we reported that soluble fractions dervided from bladder 3 to14 weeks after outlet obstruction facilitated the proliferation of cultured smooth muscle cells in the bladder of a normal rat. In order to identify the growth factors contained in the soluble fractions, we have added various growth factors to cultured normal bladder smooth muscle cells. Only basic-fibroblast growth factor (b-FGF) facilitated the growth of the smooth muscle cells. Further, using RT-PCR method, we examined which growth factors were expressed in hypertrophied bladder, and found the expression of b-FGF, nerve growth factor (NGF) and transforming growth factor-β (TGF-β). As to the reason why only b-FGF promoted growth of the cells in our study, we have to further examine the characteristics of cultured normal bladder smooth muscle cells and their growth environment.
近畿大学の論文