Characterization of Secretory Type IIA Phospholipase A_2(sPLA_2-IIA)as a Glycyrrhizin(GL)-Binding Protein and the GL-Induced Inhibition of the CK-II-Mediated Stimulation of sPLA_2-IIA Activity in Vitro

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概要

• 論文の詳細を見る

• By means of heparin-affinity and glycyrrhizin(GL)-affinity column chromatographies(HPLC), a GL-binding phospholiphase A_2(gbPLA_2)was selectively purified from the synovial fluids of patients with rheumatoid arthritis. This purified gbPLA_2 was identified as a secretory type IIA PLA_2(sPLA_2-IIA)since it was crossreacted with anti-sPLA_2-IIA serum. The activity of purified sPLA_2-IIA was inhibited by glycyrrhetinic acid(GA)and a GA derivative(oGA)in a dose-dependent manner, but it was more sensitive to GA than GL. Furthermore, it was found that(i)purified sPLA_2-IIA is phosphorylated by casein kinase II(CK-II)in vitro;(ii)this phosphorylation induces in a significant stimulation of PLA_2 activity; and(iii)oGA at one-tenth the concentration of GL inhibits the CK-II-mediated stimulation of sPLA_2-IIA activity. These results show that(i)sPLA_2-IIA is a GL-binding protein; and(ii)CK-II mediates stimulation of its PLA_2 activity in vitro.

• 公益社団法人日本薬学会の論文
• 2001-09-01

著者

• OHTSUKI Kenzo

  Laboratory of Genetical Biochemistry and Signal Biology, Graduate School of Medical Sciences, Kitasa

• TANAKA Michi

  Institute of Rheumatology, Tokyo Women's Medical University

• Ohtsuki Kenzo

  Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato

• Ohtsuki Kenzo

  Laboratory Of Genetical Biochemistry School Of Allied Health Sciences Kitasato University

• SHIMOYAMA Yoshihito

  Laboratory of Genetical Biochemistry, Kitasato University School of Allied Health Sciences

• Tanaka M

  Otsuka Pharmaceutical Co. Ltd. Tokushima Jpn

• Sakamoto Ryoko

  Laboratory Of Genetical Biochemistry School Of Allied Health Sciences Kitasato University

• Sakamoto Ryoko

  Laboratory Of Genetical Biochemistry Graduate School Of Medical Sciences Kitasato University

• AKABOSHI Tohru

  Internal Medicine, School of Medicine, Kitasato University

• Akaboshi Tohru

  Internal Medicine School Of Medicine Kitasato University

• TANAKA Michi

  Institute of Rheumatology, Tokyo Women's Medical University

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