Biochemical Characterization of 60S Acidic Ribosomal P Proteins from Porcine Liver and the Inhibition of Their Immunocomplex Formation with Sera from Systemic Lupus Erythematosus (SLE) Patients by Glycyrrhizin in Vitro

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概要

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• The three casein kinase II (CK-II) phosphate acceptors (p35,p17 and p15) in the Superdex CK-II fraction prepared from a 1.5 M NaCl extract of porcine liver were selectively purified by glycyrrhizin (GL)-affinity column chromatography (HPLC) as a heterocomplex associated with CK-II. Determination of the N-terminal amino acid sequences and immunological tests confirmed that these three CK-II phosphate acceptors belong to the family of 60S acidic ribosomal proteins (P0,P1 and P2). Three polyphenol-containing anti-oxidant compounds [catechin, epigallocatechin gallate (EGCG) and quercetin] inhibited CK-II activity (phosphorylation of these ribosomal P proteins) in a dose-dependent manner in vitro. Quercetin (ID_<50>n=approx. 50 nM) was found to be an effective CK-II inhibitor. In contrast, CK-II activity was significantly stimulated by lower doses (0.3-3 μM) of GL, but was inhibited at high doses above 30 μM. As expected, GL at high doses above 200 μM inhibited the immunocomplex formation of 60S acidic ribosomal P proteins with their specific antibodies in the sera from patients with systemic lupus erythematosus (SLE). These results suggest that (i) a GL-affinity column is useful for effective purification of 60S acidic ribosomal P proteins from various mammalian cells as a heterocomplex associated with CK-II; and (ii) a relative high dose of GL may prevent the immunocomplex formation of 60S acidic ribosomal P proteins with their specific antibodies in the sera of SLE patients.

• 公益社団法人日本薬学会の論文
• 2000-01-01

著者

• MAEKAWA Toshiro

  Laboratory of Genetical Biochemistry and Signal Biology, Graduate School of Medical Sciences, Kitasa

• OHTSUKI Kenzo

  Laboratory of Genetical Biochemistry and Signal Biology, Graduate School of Medical Sciences, Kitasa

• Munakata Hiroshi

  Department of Urology and Biochemistry, Kinki University, School of Medicine

• Ito J

  Laboratory Of Biochemistry Sagami Women's University

• Ohtsuki Kenzo

  Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato

• ITO Junko

  Laboratory of Biochemistry, Sagami Women's University

• 大久保 研之

  Department Of Clinical Chemistry And Laboratory Medicine Faculty Of Medicine Kyushu University

• Maekawa Toshiro

  Laboratory Of Genetical Biochemistry Graduate School Of Medical Sciences Kitasato University

• Maekawa Toshiro

  Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato

• MUNAKATA Haruka

  Hokkaido College of Pharmacy

• KOSUGE Seiji

  Laboratory of Genetical Biochemistry, School of Allied Health Sciences, Kitasato University

• Munakata Hiroshi

  Department Of 2nd Biochemistry Kinki University School Of Medicine

• Munakata Hiroshi

  Dept. Of Biochemistry Kinki Univ. School Of Med.

• MUTO Haruhiko

  Shizuoka Prefecture Forestry and Forest Products Research Institute

• Kosuge Seiji

  Laboratory Of Genetical Biochemistry Graduate School Of Medical Sciences Kitasato University

• Okano Tetsuro

  Clinical Immunology School Of Allied Health Sciences Kitasato University

• Ohtsuki K

  Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato

• KARINO Atsushi

  Laboratory of Genetical Biochemistry School of Allied Health Sciences, Kitasato University

• Karino A

  Laboratory Of Genetical Biochemistry School Of Allied Health Sciences Kitasato University

• Karino Atsushi

  Laboratory Of Genetical Biochemistry Kitasato University School Of Allied Health Sciences

• Munakata Hidekazu

  Department Of Biochemistry Kinki University School Of Medicine

• Maekawa Toshiro

  Laboratory For Digestive Diseases Center For Genomic Medicine Riken

• Ito Junko

  Laboratory Of Biochemistry Sagami Women's University Kitasato University

• ITO JUNKO

  Faculty of Pharmacy, Meijo University

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