Biochemical Characterization of Recombinant HIV-1 Reverse Transcriptase (rRT) as a Glycyrrhizin-Binding Protein and the CK-II-Mediated Stimulation of rRT Activity Potently Inhibited by Glycyrrhetinic Acid Derivative

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概要

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• By means of successive Mono Q and glycyrrhizin (GL)-affinity column chromatography (HPLC), recombinant HIV-1 RT (rRT) was purified to apparent homogeneity from the Superdex 200 pg fraction of the crude protein extract of E. coli BL21 transfected with pET 21a(+)/HIV-1 PR-RT. It was found that (i) rRT functioned as an effective phosphate acceptor for recombinant human casein kinase II (rhCK-II) in vitro; (ii) this phosphorylation was inhibited by anti-HIV-1 substances [a glycyrrhetinic acid derivative (oGA) and quercetin] and a high dose (100 μM) of GL; (iii) RNA-dependent DNA polymerase (RDDP) activity was stimulated about 2.5-fold after full phosphorylatioin of rRT by rhCK-II; and (iv) oGA as well as NCS-chromophore effectively prevented the CKI-II-mediated stimulation of RDDP activity. These results suggest that the anti-HIV-1 effect of oGA may be involved in the selective inhibition of the CK-II-mediated stimulation of HIV-1 RT at the cellular level.

• 公益社団法人日本薬学会の論文
• 1998-12-15

著者

• MAEKAWA Toshiro

  Laboratory of Genetical Biochemistry and Signal Biology, Graduate School of Medical Sciences, Kitasa

• OHTSUKI Kenzo

  Laboratory of Genetical Biochemistry and Signal Biology, Graduate School of Medical Sciences, Kitasa

• 原田 俊司

  Meiji College of Pharmacy

• NAGATA NOBUYUKI

  Research Laboratory, Minophagen Co.

• Nagata Nobuyuki

  Research Laboratories Of Minophagen Pharmaceutical Co. Ltd.

• Morikawa Yuko

  Section Of Virus The Kitasato Institute

• Morikawa Yuko

  Laboratory Of Food Science And Nutrition Department Of Life Style Studies School Of Human Cultures T

• Ohtsuki Kenzo

  Genetical Biochem. Kitasato Univ.

• Ohtsuki Kenzo

  Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato

• HARADA Shigeyoshi

  Laboratory of Genetical Biochemistry, School of Allied Health Sciences, Kitasato University

• HANEDA Eiji

  Laboratory of Genetical Biochemistry, School of Allied Health Sciences, Kitasato University

• MORIKAWA Yuko

  The Kitasato Institute

• Maekawa Toshiro

  Laboratory Of Genetical Biochemistry Graduate School Of Medical Sciences Kitasato University

• Maekawa Toshiro

  Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato

• Haneda Eiji

  Laboratory Of Genetical Biochemistry School Of Allied Health Sciences Kitasato University

• 永田 尚也

  Gifu Pharmaceutical University

• Ohtsuki K

  Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato

• Maekawa Toshiro

  Laboratory For Digestive Diseases Center For Genomic Medicine Riken

• Ohtsuki Kenzo

  Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato

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