Chloroplast Ribonucleoproteins (RNPs) as Phosphate Acceptors for Casein Kinase II : Purification by ssDNA-Cellulose Column Chromatography : PROTEINS, ENZYMES AND METABOLISM
スポンサーリンク
概要
- 論文の詳細を見る
Using ssDNA-cellulose column chromatography, a 34 kDa ribonucleoprotein (p34) has been purified from a 0.4 M KCl crude extract of spinach chloroplasts as an effective phosphate acceptor for casein kinase 11 (CK-II) in vitro. Monomeric and oligomeric CK-IIs were copurified with p34 by the column chromatography and the kinases were separated from p34 by means of Mono Q column chromatography. It was found that (i) the purified p34 (pI 4.9) was phosphorylated specifically by CK-II in vitro; and (ii) similar polypeptides, such as p35 (pI 4.7) and p39 (pI 4.9) in maize and p33 (pI 4.7) in liverwort, were detected as ssDNA-binding chloroplast proteins phosphorylated by CK-II in vitro. The findings suggest that (i) RNPs that function as phosphate acceptors for CK-II exist commonly in chloroplasts among plant cells; and (ii) the physiological activity of RNPs is regulated by their specific phosphorylation by CK-II in chloroplasts.
- 日本植物生理学会の論文
著者
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Nakamura Takahiro
Department of Pediatrics and Child Health, Nihon University School of Medicine
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Ohtsuki Kenzo
Genetical Biochem. Kitasato Univ.
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Nakamura Takahiro
Department Of Allergy And Immunology National Research Institute For Child Health And Development Na
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Kanekatsu Motoki
Lab. Bio.
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Kanekatu Motoki
Department Of Biological Production Faculty Of Agriculture Tokyo University Of Agriculture And Techn
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Ohtsuki K
Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato
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Nakamura T
Graduate School Of Bioagricultural Sciences Nagoya University
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KANEKATSU Motoki
Laboratory of Biology, School of Liberal Arts and Sciences, Kitasato University
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EZUMI Akiyoshi
Department of Bioscience, School of Hygienic Sciences, Kitasato University
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OHTSUKI Kenzo
Department of Bioscience, School of Hygienic Sciences, Kitasato University
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Nakamura Toshihide
Bioscience Center Nagoya University
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Ohtsuki Kenzo
Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato
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Ohtsuki Kenzo
Department Of Bacteriology Tohoku University School Of Medicine
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Nakamura Teruko
Physiological Laboratory Japan Women's University
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EZUMI Akiyoshi
Genetical Biochem., Kitasato Univ.
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Ezumi Akiyoshi
Genetical Biochem. Kitasato Univ.
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