The effects of nucleic acids on the casein kinase II catalyzed phosphorylation of a 34kDa ribonucleoprotein purified from spinach chloroplasts
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To understand biological significance of a 34kDa ribonucleoprotein (p34) in the chloroplast gene expression, effects of DNAs and RNAs on the phosphorylation of p34 by CK-II were analyzed biochemically. CK-II catalyzed phosphorylation of p34 was stimulated about 12-fold by dsDNA and 4.2-fold by poly (rG), respectively. The analysis of CD spectra revealed that DNA and RNA induced conformational change of p34. These results suggest that the conformational modification of p34 by nucleic acids results in enhancement of the affinity between the protein and CK-II. Based on the experimental results and findings of previous reports, a possible model for post-transcriptional regulation through phosphorylation of p34 by CK-II in chloroplast gene expression is proposed.
- 日本学士院の論文
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関連論文
- Chloroplast Ribonucleoproteins (RNPs) as Phosphate Acceptors for Casein Kinase II : Purification by ssDNA-Cellulose Column Chromatography : PROTEINS, ENZYMES AND METABOLISM
- Biochemical characterization of casein kinases II from chloroplasts of spinach, Spinacia oleracea
- The effects of nucleic acids on the casein kinase II catalyzed phosphorylation of a 34kDa ribonucleoprotein purified from spinach chloroplasts
- Purification of a 34 kDa ribonucleoprotein (p34) from spinach chloroplasts as an effective phosphate acceptor for casein kinase II.