Effect of Auxin and Gibberellin on Conidial Germination in Neurospora crassa II. : "Conidial Density Effect" and Auxin

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Conidial germination in liquid shake culture of Neurospora crassa was affected by the number of conidia in the medium (conidial density effect) with the optimum density being around 2×10^6 conidia/ml. The conidial density effect at 2×10^4 and 2×10^5 conidia/ml was eliminated by the addition of auxin, IAA or 2,4-D with the optimum concentrations of IAA and 2,4-D being around 10^<-6> M. IAA and 2,4-D had no effect on the effect at 2×10^7 conidia/ml. An active substance(s) for conidial germination which was related to the conidial density effect was detected in the cell-free filtrate of the germination medium, and was found to be non-dialyzable and thermolabile. At the early stage of germination, the concentration of active substance(s) in the medium increased in proportion to the conidial density and reached a supraoptimum amount for germination at 2×10^7 conidia/ml. IAA (10^<-6> M) enhanced the concentration. Endogenous auxin concentrations in filtrates of the germination media containing 2×10^5, 2×10^6 and 2×10^7 conidia/ml were 5.8×10^<-12>, 4.6×10^<-11> and 1.7×10^<-10> M IAA equivalent, respectively. The conclusion reached was that auxin may control conidial germination with mediation by the active substance(s).
日本植物生理学会の論文