A catalytic subunit of cyclic AMP-dependent protein kinase, PKAC-1, regulates asexual differentiation in Neurospora crassa

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概要

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• A cyclic AMP (cAMP)-dependent protein kinase pathway has been shown to reg-ulate growth, morphogenesis and virulence in filamentous fungi. However, the precise mechanisms of regulation through the pathway remain poorly understood. In Neurospora crassa, the cr-1 adenylate cyclase mutant exhibits colonial growth with short aerial hyphae bearing conidia, and the mcb mutant, a mutant of the regulatory subunit of cAMP-dependent protein kinase (PKA), shows the loss of growth polarity at the restrictive temperature. In the present study, we isolated mutants of the catalytic subunit of the PKA gene pkac-1 through the process of repeat-induced point mutation (RIP). PKA activity of the mutants obtained through RIP was undetectable. The genome sequence predicts two distinct catalytic subunit genes of PKA, named pkac-1 (NCU06240.1, AAF75276) and pkac-2 (NCU00682.1), as is the case in most filamentous fungi. The results sug-gest that PKAC-1 works as the major PKA in N. crassa. The phenotype of the pkac-1 mutants included colonial growth, short aerial hyphae, premature conidiation on solid medium, inappropriate conidiation in submerged culture, and increased thermotolerance. This phenotype of pkac-1 mutants resembled to that of cr-1 mutants, except that the addition of cAMP did not rescue the abnormal morphology of pkac-1 mutants. The loss of growth polarity at the restrictive temperature in the mcb mutant was suppressed by pkac-1 mutation. These results suggest that the signal transduction pathway mediated by PKAC-1 plays an important role in regulation of aerial hyphae formation, conidiation, and hyphal growth with polarity.

• 日本遺伝学会の論文
• 2005-02-01

著者

• FUJIMURA Makoto

  Faculty of Life Sciences, Toyo University

• Fujimura Makoto

  Fac. Of Life Sciences Toyo Univ.

• Fujimura M

  Faculty Of Engineering Nagasaki University

• Fujimura M

  Univ. Toyo Oura‐gun Jpn

• Yamaguchi I

  Department Of Applied Biological Chemistry The University Of Tokyo

• Fujimura M

  Fac. Of Life Sciences Toyo Univ.

• Fujimura Makoto

  The Faculty Of Engineering Nagasaki University

• YAMAGUCHI ISAMU

  Plant Science Center, RIKEN

• BANNO Shinpei

  Plant Function Research Center, Toyo University

• KIMURA Makoto

  Plant & Microbial Metabolic Engineering Research Unit, Discovery Research Institute (DRI), RIKEN

• BANNO Shinpei

  Faculty of Life Sciences, Toyo University

• OCHIAI Noriyuki

  Plant Regulation Research Center, Toyo University

• NOGUCHI Rieko

  Faculty of Life Sciences, Toyo University

• KANZAKI Sei-ichi

  College of Engineering, Kanto Gakuin University

• MURAYAMA Tadako

  College of Engineering, Kanto Gakuin University

• MOTOYAMA Takayuki

  Plant Science Center, RIKEN

• Kimura M

  Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University

• Fujimura Makoto

  Faculty Of Life Sciences Toyo University

• Yamaguchi I

  Microbial Toxicology Laboratory Riken:(present Address)environmental Plant Research Group Plant Scie

• Yamaguchi Isamu

  Laboratory For Remediation Research Plant Science Center Riken

• Yamaguchi I

  Riken The Institute Of Physical And Chemical Research

• Yamaguchi Isamu

  Plant Science Center Riken

• Yamaguchi Isomaro

  Department Of Applied Biological Chemistry University Of Tokyo

• Kimura Makoto

  Plant & Microbial Metabolic Engineering Research Unit Discovery Research Institute (dri) Riken

• Banno Shinpei

  Plant Function Research Center Toyo University

• Mimori Kouhei

  Laboratory For Remediation Research Plant Science Center Riken:department Of Engineering Toyo Univer

• Noguchi Rieko

  Faculty Of Life Sciences Toyo University

• Yamaguchi I

  Riken (the Institute Of Physical And Chemical Research) Plant Science Center

• Motoyama T

  College Of Engineering Kanto Gakuin University

• Murayama Tadako

  College Of Engineering Kanto-gakuin University

• Kanzaki Sei-ichi

  College Of Engineering Kanto Gakuin University

• Ochiai Noriyuki

  Plant And Microbial Metabolic Engineering Research Unit Discovery Research Institute (dri) Riken

• KIMURA Makoto

  Plant & Microbial Metabolic Engineering Research Unit, Discovery Research Institute (DRI), RIKEN

• FUJIMURA Makoto

  Faculty of Life Science, University of Toyo

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