Expression of a Functional Single-chain Antibody against GA_<24/19> in Transgenic Tobacco

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概要

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• An anti-gibberellin A_<24/19> single-chain Fv gene was constructed from γ and κ genes cloned from a hybridoma cell line producing monoclonal antibody against gibberellin A_<24/19>, biosynthetic precursors of gibberellin A_<4/1> which are biologically active per se. The single-chain Fv gene was introduced into tobacco plants after the binding activity of the single-chain Fv expressed in Escherichia coli was confirmed. When the single-chain Fv expression is targeted to endoplasmic reticulum, the plants could accumulate the single-chain Fv protein with the antigen binding activity up to 3.6% of the total soluble protein. On the other hand, when the expression is targeted to cytosol, accumulation of the single-chain Fv protein was not detected at all. The dwarf phenotype of the transgenic plants expressing the single-chain Fv protein, together with the preliminary analytical data indicating a decreased level of gibberellin A_1 in the dwarf transgenics, suggested that the single-chain Fv decreased the concentration of bioactive gibberellins by trapping and inhibiting the metabolism of gibberellin A_24 and/or A_19 to gibberellin A_4 and/or A_1.

• 社団法人日本農芸化学会の論文
• 1999-04-23

著者

• 吉田 静夫

  北海道大学低温科学研究所

• SHIMADA Naoki

  Department of Preventive Medicine and Public Health, School of Medicine, Keio University

• SUZUKI Yoshihito

  Department of Applied Biological Chemistry, The University of Tokyo

• MUROFUSHI Noboru

  Faculty of Bioresource Sciences, Akita Prefectural University

• YAMAGUCHI Isomaro

  Department of Applied Biological Chemistry, The University of Tokyo

• NAKAJIMA Masatoshi

  Department of Applied Biological Sciences, The University of Tokyo

• Yamaguchi I

  Department Of Applied Biological Chemistry The University Of Tokyo

• MUROFUSHI Noboru

  Department of Biotechnology, Akita Prefectural University

• Shimada Naoki

  Department Of Applied Biological Chemistry Division Of Agriculture And Agricultural Life Sciences Th

• Yoshida S

  Laboratory Of Cancer Cell Biology Research Institute For Disease Mechanism And Control Nagoya Univer

• Maejima Noboru

  Department Of Biotechnology Akita Prefectural University

• Suzuki Y

  Department Of Applied Biological Chemistry The University Of Tokyo

• Suzuki Yoshihito

  Dep. Of Applied Biological Chemistry Univ. Of Tokyo

• Suzuki Y

  Department Of Global Epidemiology Hokkaido University Research Center For Zoonosis Control

• Conrad Udo

  Institutfur Pflanzengenetik und kulturpflanzenforschung

• Yamaguchi Isomaro

  Department Of Applied Biological Chemistry The University Of Tokyo

• Yoshida Shigeo

  Plant Functions Lab. The Inst. Of Phys. And Chem. Res. (riken)

• Conrad Udo

  Institute Of Plant Genetics And Crop Plant Research

• Murofushi Noboru

  Faculty Of Bioresource Sciences Akita Prefectural University

• Murofushi Noboru

  Department Of Agricultural Chemistry The University Of Tokyo

• Suzuki Yoshihito

  Department Of Applied Biological Chemistry The University Of Tokyo

• Suzuki Yoshihito

  The Institute Of Enology And Viticulture Yamanashi University

• Yamaguchi Isomaro

  Department Of Agricultural Chemistry Faculty Of Agricultural Science University Of Tokyo

• Nakajima Masatoshi

  Department Of Applied Biological Chemistry The University Of Tokyo

• Suzuki Y

  Grad. Sch. Bioagric. Sci. Nagoya Univ.

• Yamaguchi I

  Department Of Applied Biological Chemistry University Of Tokyo

• Nakayama M

  Juntendo Univ. School Of Medicine Tokyo Jpn

• Nakajima Masatoshi

  Department Of Applied Biological Chemistry University Of Tokyo

• Suzuki Y

  Research Institute For Bioresources Okayama University

• Nakayama M

  Dept. Biol. Sci. Fac. Biosci. Biotechnol. Tokyo Inst. Technol.

• Nonaka M

  Department Of Applied Biological Chemistry The University Of Tokyo

• Suzuki Yoshihito

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Yamaguchi Isomaro

  Dept. Appl. Biol. Chem. Tokyo Univ.

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