Enzyme Inhibitors to Increase Poly-3-hydroxybutyrate Production by Transgenic Tobacco(Biochemistry & Molecular Biology)

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概要

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• Chemical regulation of secondary-metabolite synthesis was investigated through the improvement of poly-3-hydroxybutyrate (PHB) production in transgenic tobacco plants by the use of enzyme inhibitors. Two tobacco lines, BC3 and rCAB8, that produce PHB in both the cytosol and plastids were used. An acetyl-CoA carboxylase inhibitor, D- (+) -Quizalofop-ethyl, increased PHB accumulation in both lines 2-fold. The accumulation rate of plastidial PHB in the rCABS line was 2.5-fold higher than that of cytosolic PHB in the BC3 line. A specific inhibitor of 3-hydroxy-3-methylglutaryl-CoA reductase, mevastatin, also increased PHB accumulation but only in the BC3 line. These results indicated that chemical regulation of the native metabolic flows by the specific enzyme inhibitors increased secondary-metabolite production in the transgenic tobacco plants we used.

• 社団法人日本農芸化学会の論文
• 2002-12-23

著者

• 吉田 静夫

  北大低温研

• 吉田 静夫

  Discovery Res. Inst. Riken

• 吉田 静夫

  北海道大学低温科学研究所

• 吉田 静夫

  北大・低温研

• Yoshida S

  Riken Kanagawa Jpn

• Suzuki Yoshikatsu

  Plant Science Center Riken Institute

• Kawano H

  Extremobiosphere Research Center (xbr) Japan Agency For Marine-earth Science And Technology (jamstec

• YOSHIDA Shigeo

  Institute of Physical and Chemical Research

• Nakamura Hidemitsu

  Department Of Plant Biotechnology National Institute Of Agrobiological Sciences

• YAMAGUCHI Isamu

  RIKEN, The Institute of Physical and Chemical Research

• ARAI Yuko

  Microbial Toxicology Laboratory, RIKEN Institute

• SUZUKI Yoshikatsu

  RIKEN(The Institute of Physical and Chemical Research)

• ARAI Yuko

  RIKEN Institute

• NAKASHITA Hideo

  RIKEN Institute

• DOI Yoshiharu

  RIKEN Institute

• Nakashita Hideo

  Environmental Molecular Biology Laboratory Discovery Research Institute Riken

• Nakashita Hideo

  Riken Advanced Science Institute

• KURANO Minoru

  RIKEN Institute (The Institute of Physical and Chemical Research)

• USAMI Ron

  Toyo University

• HORIKOSHI Kohki

  Toyo University

• Suzuki Yoshihito

  Dep. Of Applied Biological Chemistry Univ. Of Tokyo

• Yamaguchi I

  Microbial Toxicology Laboratory Riken:(present Address)environmental Plant Research Group Plant Scie

• Yamaguchi Isomaro

  Department Of Applied Biological Chemistry The University Of Tokyo

• Suzuki Yoshikatsu

  Riken (the Institute Of Physical And Chemical Research)

• Yamaguchi Isomaro

  Department Of Applied Biological Chemistry University Of Tokyo

• Yamaguchi I

  Department Of Applied Biological Chemistry University Of Tokyo

• Usami R

  Department Of Applied Chemistry Faculty Of Engineering Toyo University

• Usami R

  Toyo Univ. Saitama Jpn

• Arai Y

  Microbial Toxicology Laboratory Riken Institute:graduate School Of Science And Engineering Saitama U

• Horikoshi K

  Extremobiosphere Res. Center (xbr) Japan Agency For Marine-earth Sci. And Technol. (jamstec)

• Kurano Minoru

  Riken Institute (the Institute Of Physical And Chemical Research):toyo University

• Doi Yoshiharu

  Polymer Chemistry Laboratory Riken Institute

• Yamaguchi I

  The Institute Of Physical And Chemical Research (riken)

• Shimada Yukihisa

  Plant Science Center Riken

• Yamaguchi Isamu

  Riken Institute (the Institute Of Physical And Chemical Research) Microbial Toxicology Laboratory

• Usami Ron

  Graduate School Of Interdisciplinary New Science Toyo University

• Yoshida Saiko

  Department Of Biological Sciences Graduate School Of Science University Of Tokyo

• Yoshida Shigeo

  Plant Functions Lab. The Inst. Of Physical And Chemical Res.

• Yoshida Shigeo

  Inst. Biol. Sci. Tsukuba Univ.

• Yamaguchi Isomaro

  Dept. Appl. Biol. Chem. Tokyo Univ.

• Yamaguchi Isamu

  RIKEN (The Institute of Physical and Chemical Research) Plant Science Center

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