トドマツ種子の発芽および低温湿層処理の生化学(第1報) : トドマツ種子の低温湿層処理の過程におけるauxinとinhibitorの消長について
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概要
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トドマツ種子中のauxinとinhibitorが低温湿層処理の過程でどのような消長をたどるかを究明するために以下の実験を行なつた。低温湿層処理を施した種子中のauxinとinhibitorは70%のエタノールで20時間, 0℃で抽出した。抽出物は, isopropanol : ammonia : water, 10 : 1 : 1 (in vol.)でPaper chromatographyで分離し, Avena straight growth testで生物試験したところ, Rf 0.12にあたらしい生長促進物質が検出された。この物質はエールリツヒ試薬に陽性であつた。また, この物質は低温湿層処理が進むにつれて漸次増加することがわかつた。抽出物を1週間冷蔵庫に放置したのち再度精製し, 樹脂様物質を完全に除去したものについて同様に生物試験を行なつたところ, β-Indolylacetic acid (IAA)と同一のRfのところに生長促進物質が現われ, 未知の生長促進物質は逆に減少した。これはRf 0.12の未知生長促進物質がIAAに変化したものと考えられ, この未知生長促進物質は, あるいは, Indole化合物で, 容易にIAAに変化し得る性質のものと推定できる。
- 日本森林学会の論文
- 1960-07-25
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