Preparation and Application of Anti-idiotypic Antibody against Anti-gibberellin A_4 Antibody

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概要

• 論文の詳細を見る

• A monoclonal anti-idiotypic antibody was raised against anti-gibberellin A_4 (GA_4) antibody, which recognizes biologically active gibberellins such as GA_1 and GA_4 specifically. Amino acid sequences of variable regions of both anti-GA_4 and anti-idiotypic antibodies were analyzed. By using the property of the anti-idiotypic antibody to compete with GA_<1/4> in binding to the anti-GA_4 antibody, we successfully applied the anti-idiotypic antibody to ELISA as a tracer for measuring GA_<1/4>. The single-chain Fv (scFv) gene of the anti-idiotypic antibody was constructed, and scFv expressed in E. coli showed binding activity to anti-GA_4 antibody. These results suggest the possible application of anti-idiotypic antibody as a handy and stable source of an enzymatic tracer for ELISA by production of fusion protein of the scFv and an appropriate enzyme.

• 社団法人日本農芸化学会の論文
• 1999-04-23

著者

• 吉田 静夫

  北海道大学低温科学研究所

• SHIMADA Naoki

  Department of Preventive Medicine and Public Health, School of Medicine, Keio University

• SUZUKI Yoshihito

  Department of Applied Biological Chemistry, The University of Tokyo

• MUROFUSHI Noboru

  Faculty of Bioresource Sciences, Akita Prefectural University

• YAMAGUCHI Isomaro

  Department of Applied Biological Chemistry, The University of Tokyo

• NAKAJIMA Masatoshi

  Department of Applied Biological Sciences, The University of Tokyo

• Yamaguchi I

  Department Of Applied Biological Chemistry The University Of Tokyo

• MUROFUSHI Noboru

  Department of Biotechnology, Akita Prefectural University

• Shimada Naoki

  Department Of Applied Biological Chemistry Division Of Agriculture And Agricultural Life Sciences Th

• Yoshida S

  Laboratory Of Cancer Cell Biology Research Institute For Disease Mechanism And Control Nagoya Univer

• Maejima Noboru

  Department Of Biotechnology Akita Prefectural University

• Suzuki Y

  Department Of Applied Biological Chemistry The University Of Tokyo

• Suzuki Yoshihito

  Dep. Of Applied Biological Chemistry Univ. Of Tokyo

• Suzuki Y

  Department Of Global Epidemiology Hokkaido University Research Center For Zoonosis Control

• Yamaguchi Isomaro

  Department Of Applied Biological Chemistry The University Of Tokyo

• Yoshida Shigeo

  Plant Functions Lab. The Inst. Of Phys. And Chem. Res. (riken)

• Murofushi Noboru

  Faculty Of Bioresource Sciences Akita Prefectural University

• Murofushi Noboru

  Department Of Agricultural Chemistry The University Of Tokyo

• Suzuki Yoshihito

  Department Of Applied Biological Chemistry The University Of Tokyo

• Suzuki Yoshihito

  The Institute Of Enology And Viticulture Yamanashi University

• Yamaguchi Isomaro

  Department Of Agricultural Chemistry Faculty Of Agricultural Science University Of Tokyo

• Nakajima Masatoshi

  Department Of Applied Biological Chemistry The University Of Tokyo

• Suzuki Y

  Grad. Sch. Bioagric. Sci. Nagoya Univ.

• Yamaguchi I

  Department Of Applied Biological Chemistry University Of Tokyo

• Nakayama M

  Juntendo Univ. School Of Medicine Tokyo Jpn

• Nakajima Masatoshi

  Department Of Applied Biological Chemistry University Of Tokyo

• Suzuki Y

  Research Institute For Bioresources Okayama University

• Nakayama M

  Dept. Biol. Sci. Fac. Biosci. Biotechnol. Tokyo Inst. Technol.

• NIWA Rimpei

  Department of Applied Biological Chemistry, Division of Agriculture and Agricultural Life Sciences,

• YOKOI Michinori

  Department of Applied Biological Chemistry, Division of Agriculture and Agricultural Life Sciences,

• Niwa Rimpei

  Department Of Applied Biological Chemistry Division Of Agriculture And Agricultural Life Sciences Th

• Yokoi Michinori

  Department Of Applied Biological Chemistry Division Of Agriculture And Agricultural Life Sciences Th

• Nonaka M

  Department Of Applied Biological Chemistry The University Of Tokyo

• Suzuki Yoshihito

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Yamaguchi Isomaro

  Dept. Appl. Biol. Chem. Tokyo Univ.

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