Expression Pattern of the CsPK3 Auxin-Responsive Protein Kinase Gene

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概要

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• We have previously cloned a cDNA of a putative serine/threonine protein kinase gene named CsPK3 from cucumber, the mRNA level of which was up-regulated by auxin and down-regulated by light irradiation. To examine the CsPK3 gene expression in detail, we cloned a genomic DNA of CsPK3 gene and made transgenic tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) plants containing the fused CsPK3 promoter-β-glucuronidase gene. The β-glucuronidase expression was detected in the shoot apex, vascular tissues, and the outermost layer of cortex. The histological distribution of CsPK3 mRNA in cucumber seedlings was supported by in situ hybridization, where the positive signals were observed in similar tissues as those observed by β-glucuronidase staining. The responsiveness of the CsPK3 gene to auxin and light was also confirmed for β-glucuronidase activity. The pattern of β-glucuronldase staining changed during the development of the tobacco seedlings. The results of our experiment showed that CsPK3 was expressed in a wide variety of tissues and cells in which the developmental and growth controls by auxin are suggested.

• 2001-03-23

著者

• 吉田 静夫

  Discovery Res. Inst. Riken

• 吉田 静夫

  北海道大学低温科学研究所

• 吉田 静夫

  北大・低温研

• Yoshida S

  Riken Kanagawa Jpn

• YAMANE Hisakazu

  Biotechnology Research Center, The University of Tokyo

• CHONO Makiko

  Department of Wheat and Barley, National Institute of Crop Science, National Agricultural Research O

• SUZUKI Yoshihito

  Department of Applied Biological Chemistry, The University of Tokyo

• NEMOTO Keisuke

  Asian Natural Environmental Science Center, The University of Tokyo

• MUROFUSHI Noboru

  Faculty of Bioresource Sciences, Akita Prefectural University

• YAMAGUCHI Isomaro

  Department of Applied Biological Chemistry, The University of Tokyo

• Chono Makiko

  Department Of Wheat And Barley Research National Institute Of Crop Science

• Chono Makiko

  National Institute Of Crop Science (nics)

• Yoshida S

  Laboratory Of Cancer Cell Biology Research Institute For Disease Mechanism And Control Nagoya Univer

• Nemoto Keisuke

  Asian Natural Environmental Science Center The Univ. Of Tokyo

• Maejima Noboru

  Department Of Biotechnology Akita Prefectural University

• Suzuki Y

  Department Of Applied Biological Chemistry The University Of Tokyo

• Suzuki Yoshihito

  Dep. Of Applied Biological Chemistry Univ. Of Tokyo

• Nemoto K

  Asian Natural Environmental Science Center The University Of Tokyo

• Suzuki Y

  Department Of Global Epidemiology Hokkaido University Research Center For Zoonosis Control

• Yamaguchi Isomaro

  Department Of Applied Biological Chemistry The University Of Tokyo

• Yoshida Shigeo

  Plant Functions Lab. The Inst. Of Phys. And Chem. Res. (riken)

• Yamane Hisakazu

  Biotechnology Res. Center The Univ. Of Tokyo

• Yamane Hisakazu

  Biotechnology Res. Center Univ. Of Tokyo

• Yamane Hisakazu

  Biotech. Res. Ctr. The Univ. Of Tokyo

• Murofushi Noboru

  Faculty Of Bioresource Sciences Akita Prefectural University

• Suzuki Yoshihito

  Department Of Applied Biological Chemistry The University Of Tokyo

• Suzuki Yoshihito

  The Institute Of Enology And Viticulture Yamanashi University

• Yamaguchi Isomaro

  Department Of Agricultural Chemistry Faculty Of Agricultural Science University Of Tokyo

• Suzuki Y

  Grad. Sch. Bioagric. Sci. Nagoya Univ.

• Yamaguchi I

  Department Of Applied Biological Chemistry University Of Tokyo

• Suzuki Y

  Research Institute For Bioresources Okayama University

• Yamaguchi I

  The Institute Of Physical And Chemical Research (riken)

• Shimada Yukihisa

  Plant Science Center Riken

• Yoshida Saiko

  Department Of Biological Sciences Graduate School Of Science University Of Tokyo

• Suzuki Yoshihito

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Yada H

  Biotechnology Research Center University Of Tokyo

• Chono Makiko

  Naro Institute Of Crop Science

• Yamaguchi Isomaro

  Dept. Appl. Biol. Chem. Tokyo Univ.

• YAMANE Hisakazu

  Biotech. Res. Ctr. , The Univ. of Tokyo

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