Comparisons of Plasma Membranes from Shoots and Roots of Winter Rye (Secale cereale L. cv. Puma) : Polypeptide Composition, ATPase Activity and Specific Naphthylphthalamic Acid Binding Capacity

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Polypeptide compositions, ATPase characteristics, and the N-1-naphthylphthalamic acid binding capacity of plasma membranes prepared from winter rye (Secale cereale L. cv. Puma) shoots and roots were examined and compared. Some unique polypeptides were revealed in each plasma membrane by one- and two-dimensional slab gel electrophoresis. A difference was also detected in glycopeptide compositions. The plasma membranes from both organs contained Mg^<2+>_stimulated ATPase exhibiting slightly different properties in the divalent cation specificity and the kinetic constants. The ATPase activities from both organs showed a similar optimum pH around 6.5, simple Michaelis-Menten saturation with increasing ATP-Mg concentrations, and little K^+-stimulation at the optimum pH. Both ATPases were inhibited by orthovanadate, however, the degree of inhibition was a little different in each membrane sample. The specific N-1-naphthylphthalamic acid binding capacity in the shoot plasma membrane was 2.6-fold higher than that in the root plasma membrane. These results suggest that polypeptide compositions of plasma membranes vary corresponding with a difference in the physiological functions of plasma membranes between shoots and roots of winter rye.
日本植物生理学会の論文