Genomic Cloning of Ribonucleases in Nicotiana glutinosa Leaves, as Induced in Response to Wounding or to TMV-Infection, and Characterization of Their Promoters(Biochemistry & Molecular Biology)

スポンサーリンク

概要

• 論文の詳細を見る

• We previously cloned two distinct cDNA clones, NGR1 and NGR3, encoding S-like ribonucleases (RNases) induced by wounding and tobacco mosaic virus (TMV) infection, respectively, in Nicotiana glutinosa leaves. To gain insight into the regulatory mechanism of the RNase genes, we analyzed nucleotide sequences of the genes ngr1 (4.1 kbp) and ngr3 (5.3 kbp), containing their structural genes as well as 5'-flanking regions. The ngr1 gene is organized in three exons with two intervening introns, and ngr3 has four exons interrupted by three introns. Primer extension analyses localized single transcription initiation sites at -32 and -99 upstream of the translation initiation codons ATG in the genes ngr1 and ngr3, respectively. The β-glucuronidase (GUS) reporter gene analysis with serial 5'-deletion mutants as well as a gel shift assay defined the wound-responsive region at residues -509 to -288 in gene ngr1 and a TMV-responsive region at the residues -401 to -174 in ngr3, respectively. Sequence search using PLACE and PlantCARE data bases showed that a wound-responsive element : the WUN-motif, occurs within the wound-responsive region in ngr1, while ngr3 contains several potential cis-regulating elements, such as the elicitor responsiveness element : the W-box, a TMV responsive element : GT1, and the WUN-motif at positions between -401 and -174. These findings suggested that some of these cis-elements may be involved in inducible expressions of ngrl and ngr3. Furthermore, the gel shift assay suggested that the dissociation of protein factor(s) upon TMV-infection from the regulatory region may cause an inducible expression of ngr3.

• 社団法人日本農芸化学会の論文
• 2003-12-23

著者

• KIMURA Makoto

  Laboratory of Structural Biology, Graduate School of Systems Life Sciences, Kyushu University

• Hada Kazumasa

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat

• KOUZUMA Yoshiaki

  Laboratory of Biochemistry, Faculty of Agriculture, Graduate School, Kyushu University

• Kimura M

  Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University

• HAYASHI Takeshi

  Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Grad

• KOBAYASHI Daiki

  Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Grad

• KARIU Tohru

  Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Grad

• TAHARA Maino

  Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Grad

• Kobayashi Daiki

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat

• Hayashi Takeshi

  National Institute Of Industrial Health

• Hayashi T

  Kobe Univ. Kobe Jpn

• Kariu Tohru

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat

• Tahara M

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat

• Tahara Maino

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Kyushu

• Kouzuma Yoshiaki

  Laboratory Of Food Molecular Functionality College Of Agriculture Ibaraki University

• Kouzuma Yoshiaki

  Laboratory Of Food Functionality School Of Agriculture Ibaraki University

• Kawabe Masato

  United Graduate School Of Agricultural Science Tokyo University Of Agriculture And Technology (tuat)

• Kimura Makoto

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agr

• Hada Kazumasa

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat

• Kimura Makoto

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat

• Hayashi Takeshi

  Laboratory Of Animal Genetics Graduate School Of Biosphere Science Hiroshima University

• Kimura Makoto

  Digital Human Research Center Aist Japan

• Kimura Makoto

  Laboratory Of Biochemistry Department Of Agricultural Chemistry Faculty And Graduate School Of Agric

• Makoto KIMURA

  Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University

• KOBAYASHI Daiki

  Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University

• Maino TAHARA

  Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University

• Kazumasa HADA

  Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University

• Yoshiaki KOUZUMA

  Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University

• KARIU Tohru

  Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University

• HAYASHI Takeshi

  Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University

関連論文

• Archaeal Homologs of Human RNase P Protein Pairs Pop5 with Rpp30 and Rpp21 with Rpp29 Work on Distinct Functional Domains of the RNA Subunit
• Crystal Structure of hyperthermophilic Archaeal Initiation Factor 5A : A Homologue of Eukaryotic Initiation Factor 5A (eIF-5A)
• The Structure of the Archaebacterial Ribosomal Protein S7 and Its Possible Interaction with 16S rRNA^1
• Characterization of Functional Domains of the Hemolytic Lectin CEL-III from the Marine Invertebrate Cucumaria echinata
• Involvement of OS-2 MAP kinase in regulation of the large-subunit catalases CAT-1 and CAT-3 in Neurospora crassa
• Survey of mutations of a histidine kinase gene BcOS1 in dicarboximide-resistant field isolates of Botrytis cinerea
• A catalytic subunit of cyclic AMP-dependent protein kinase, PKAC-1, regulates asexual differentiation in Neurospora crassa
• Expression in Cereal Plants of Genes That Inactivate Fusarium Mycotoxins(Biochemistry & Molecular Biology)
• Proteome Analysis of Wheat Lemma(Biochemistry & Molecular Biology)
• Cytotoxicity Induced by Erythrina variegata Serine Proteinase Inhibitors in Tumor Hematopoietic Stem Cell Lines
• Methanogenic archaeal communities developed in paddy fields in the Kojima Bay polder, estimated by denaturing gradient gel electrophoresis, real-time PCR and sequencing analyses(Soil Biology)
• Structural Changes in Ribonuclease P RNA in the Hyperthermophilic Archaeon Pyrococcus horikoshii OT3 Induced on Interaction with Proteins
• Crystal Structure and Functional Analysis of an Archaeal Chromatin Protein Alba from the Hyperthermophilic Archaeon Pyrococcus horikoshii OT3
• Origin and Properties of Humus in the Subsoil of Irrigated Rice Paddles : IV. Effect of Water Percolation on the Binding Type of Humus in a Submerged Plow Layer Soil
• Comparison of Humus Composition in the Subsoil of Japanese Paddy and Upland Fields
• Origin and Properties of Humus in the Subsoil of Irrigated Rice Paddies : III. Changes in Binding Type of Humus in Submerged Plow Layer Soil
• Origin and Properties of Humus in the Subsoil of Irrigated Rice Paddies : II. Effects of Subsoil Constituents and Drainage on the Accumulation of Organic Matter Leached from the Plow Layer Soil
• Origin and Properties of Humus in the Subsoil of Irrigated Rice Paddies : I. Leaching of Organic Matter from Plow Layer Soil and Accumulation in Subsoil
• Genomic Cloning of Ribonucleases in Nicotiana glutinosa Leaves, as Induced in Response to Wounding or to TMV-Infection, and Characterization of Their Promoters(Biochemistry & Molecular Biology)
• Contribution of Conserved Asn Residues to the Inhibitory Activities of Kunitz-Type Protease Inhibitors from Plants
• Protein Engineering of Novel Proteinase Inhibitors and Their Effects on the Growth of Spodoptera exigua Larvae(Biochemistry & Molecular Biology)
• Calcium Ions Stabilize a Protein Structure of Hemolytic Lectin CEL-III from Marine Invertebrate Cucumaria echinata
• Molecular Cloning and Functional Expression of cDNA Encoding the Cysteine Proteinase Inhibitor Sca from Sunflower Seeds
• Conformation of the Primary Binding Loop Folded through an Itramolecular Iteraction Contributes to the Strong Chymotrpsin Inhibitory Activity of the Chymotrypsin Inhibitor from Erythrina variegata Seeds
• Molecular Cloning, Functional Expression, and Mutagenesis of cDNA Encoding a Cysteine Proteinase Inhibitor from Sunflower Seeds
• Chymotrypsin Inhibitor from Erythrina variegata Seeds : Involvement of Amino Acid Residues within the Primary Binding Loop in Potent Inhibitory Activity toward Chymotrypsin
• Cloning, Expression, and Mutagenesis of Trypsin Inhibitor ETIb from Erythrina variegata Seeds
• The Tissue-Type Plasminogen Acitvator Inhibitor ETIa from Erythrina variegata: Structural Basis for the Inhibitory Activity by Cloning, Expression, and Mutagenesis of the cDNA Encoding ETIa
• Molecular Cloning of cDNA That Encodes Chymotrypsin Inhibitor ECI from Erythrina variegata Seeds and Its Expression in Escherichia coli
• Inhibitory Potency of Erythrina variegata Proteinase Inhibitors toward Serine Proteinases in the Blood Coagulation and Fibrinolytic Systems
• Purification, Characterization, and Sequencing of Two Cysteine Proteinase Inhibitors, Sca and Scb, from Sunflower (Helianthus annuus) Seeds
• Amino Acid Sequence of Chymotrypsin Inhibitor ECI from the Seeds of Erythrina variegata (LINN.) var. Orientalis
• Isolation and Primary Structure of Proteinase Inhibitors from Erythrina variegata (LINN.) var. Orientalis Seeds
• On the Interaction of Ribosomal Protein L5 with 5S rRNA(Biochemistry & Molecular Biology)
• Arginine-55 in the β-Arm is Essential for the Activity of DNA-Binding Protein HU from Bacillus stearothermophilus
• Contribution of a Salt Bridge to the Thermostability of DNA Binding Proten HU from Bacillus stearothermophilus Determined by Site-Directed Mutagenesis
• Characterization of the Archaeal Ribonuclease P Proteins from Pyrococcus horikoshii OT3
• Effect of elevated atmospheric CO_2 concentration on ammonia oxidizing bacteria communities inhabiting in rice roots(Soil Biology)
• A Wheat Xylanase Inhibitor Gene, Xip-I, but Not Taxi-I, Is Significantly Induced by Biotic and Abiotic Signals That Trigger Plant Defense
• New TAXI-type Xylanase Inhibitor Genes are Inducible by Pathogens and Wounding in Hexaploid Wheat
• Biotransformation of the Mycotoxin, Zearalenone, to a Non-estrogenic Compound by a Fungal Strain of Clonostachys sp.(Organic Chemistry)
• EFFECTS OF TRICHOTHECENE MYCOTOXIN ON GROWTH AND MORPHOLOGY OF ARABIDOPSIS AND WHEAT
• Intracellular and extracellular free N-glycans produced by plant cells: occurrence of unusual plant complex-type free N-glycans in extracellular spaces
• Tumor Antigen Occurs in N-Glycan of Royal Jelly Glycoproteins : Honeybee Cells Synthesize T-Antigen Unit in N-Glycan Moiety
• Occurrence of Lewis a Epitope in N-Glycans of a Glycoallergen, Jun a 1, from Mountain Cedar (Juniperus ashei) Pollen
• Structural Evidence for Endocrine Disruptor Bisphenol A Binding to Human Nuclear Receptor ERRγ
• Cloning of the Gene Encoding DNA Binding Protein HU from Bacillus stearothermophilus and Its Expression in Escherichia coli
• The Evolution of the Phenylacetic Acid Degradation Pathway in Bacteria
• Vertical changes in bacterial and archaeal communities with soil depth in Japanese paddy fields(Soil Biology)
• Molecular characterization of methane-oxidizing bacteria associated with rice straw decomposition in a rice field(Soil Biology)
• Primary Structure of 6.5k-Arginine/Glutamate-rich Polypeptide from the Seeds of Sponge Gourd (Luffa cylindrica)
• The Action of Rice Branching Enzyme I (BEI) on Starches
• Suppression of Ralstonia solanacearum in Soil Following Colonization by Other Strains of R. solanacearum
• Morphology, host range and phylogenetic diversity of Sphingomonas phages in the floodwater of a Japanese paddy field(Soil Biology)
• Abundance of bacteriophages of common heterotrophic bacteria in the floodwater of a Japanese paddy field(Soil Biology)
• Seasonal variations in the abundance of virus-like particles and bacteria in the floodwater of a Japanese paddy field(Soil Biology)
• Requirement for C-terminal Extension to the RNA Binding Domain for Efficient RNA Binding by Ribosomal Protein L2(Biochemistry & Molecular Biology)
• Comprehensive Analysis of Wound-inducible Genes from the Nicotiana glutinosa Leaves Using a Full-length cDNA Microarray
• 5-8 Distribution of Photosynthetically Fixed C in Irrigated Rice : I. Contribution to Soil Dissolved Organic C and Microbial Biomass
• Effect of Rice Straw Application on CH_4 Emission from Paddy Fields : IV. Influence of Rice Straw Incorporated during the Previous Cropping Period
• Effect of Rice Straw Application on CH_4 Emission from Paddy Fields : III. Effect of Incorporation Site of Rice Straw on CH_4 Emission Rates and Their Variation among Shoots of a Rice Plant
• Methane Production and Its Fate in Paddy Fields : V. Fate of Methane Remaining in Paddy Soil at Harvesting Stage
• Activities of Soil Enzymes in Different Land-Use Systems in Middle Terrace Areas of Lampung Province, South Sumatra, Indonesia
• The Contribution of Peripheral Stem-Loops to the Catalytic Activity of Archaeal RNase P RNA from Pyrococcus horikoshii OT3
• Contribution of Gln9 and Phe8O to Substrate Binding in Ribonuclease MC1 from Bitter Gourd Seeds
• Amino Acids Conserved at the C-Terminal Half of the Ribonuclease T2 Family Contribute to Protein Stability of the Enzymes
• Expression and Mutational Analysis of Amino Acid Residues Involved in Catalytic Activity in a Ribonuclease MC1 from the Seeds of Bitter Gourd
• Molecular analysis of the ammonia oxidizing bacterial community in the surface soil layer of a Japanese paddy field(Soil Biology)
• Identification of Nucleotide Residues Essential for RNase P Activity from the Hyperthermophilic Archaeon Pyrococcus horikoshii OT3
• Observation of Microbial Colonization on the Surface of Rice Roots along with Their Development and Degradation
• Comparison of microbial community structure between soils suppressive and conducive to Fusarium-wilt of radish
• Epibiotic Microorganisms Associated with Microcrustaceans in Overlying Water of Philippine Paddy Fields
• Impact of Fumigation with Metam Sodium upon Soil Microbial Community Structure in Two Japanese Soils
• Seasonal Variation of Microcrustaceans and Microbial Flora on Their Surface in the Overlying Water of a Japanese Paddy Field
• Epibiotic Bacteria Associated with Microcrustaceans in the Overlying Water of Paddy Fields
• Isolation and Characterization of Bacteria Responsible for the Suppression of Fusarium oxysporum f. sp. raphani on the Host Rhizoplane
• Mechanisms of Suppression of Fusarium oxysporum f. sp. raphani in Soils So-Called Suppressive to Fusarium-Wilt of Radish
• Biochemical and Crystallographic Characterization of the Starch Branching Enzyme I (BEI) from Oryza sativa L
• Molecular Cloning of cDNAs Encoding Ribonuclease-related Proteins in Nicotiana glutinosa Leaves, as Induced in Response to Wounding or to TMV-infection(Biochemistry & Molecular Biology)
• Expression of Nicotiana glutinosa Ribonucleases in Escherichia coli(Biochemistry & Molecular Biology)
• Crystal Structures of the Nicotiana glutinosa Ribonuclease NT in Complex with Nucleoside Monophosphates
• The Action of Rice Branching Enzyme I (BEI) on Starches
• Biochemical and Crystallographic Characterization of the Starch Branching Enzyme I (BEI) from Oryza sativa L
• The C-Terminal Portion of an Archaeal Toxin, aRelE, Plays a Crucial Role in Protein Synthesis Inhibition
• Crystal Structure and Functional Analysis of an Archaeal Chromatin Protein Alba from the Hyperthermophilic Archaeon Pyrococcus horikoshii OT3
• Phenotypic Characterization of a Mutant of Burkholderia cepacia MRT11 Defective in Rhizosphere Competence
• Effects of Soil Temperature and Anaerobiosis on Degradation of Biodegradable Plastics in Soil and Their Degrading Microorganisms
• Characterization of ammonia oxidizing bacteria associated with weeds in a Japanese paddy field using amoA gene fragments(Soil Biology)
• 2P-019 マウス硫酸転移酵素SULT5A1のX線結晶構造解析(蛋白質・構造(2),第46回日本生物物理学会年会)
• The Complete Amino Acid Sequences of the B-Chains of Abrin-a and Abrin-b, Toxic Proteins from the Seeds of Abrus precatorius
• Isolation and Molecular Characterization of Four Arginine/Glutamate Rich Polypeptides from the Seeds of Sponge Gourd (Luffa cylindrica)
• Structural Changes in Ribonuclease P RNA in the Hyperthermophilic Archaeon Pyrococcus horikoshii OT3 Induced on Interaction with Proteins
• Purification, Characterization, and cDNA Cloning of a Bowman-Birk Type Trypsin Inhibitor from Apios americana Medikus Tubers
• Isolation and Properties of Antioxidative Peptides from Water-Soluble Royal Jelly Protein Hydrolysate
• Molecular Cloning, Functional Expression, and Characterization of Isolectin Genes of Hemolytic Lectin CEL-III from the Marine Invertebrate Cucumaria echinata
• Characterization of Functional Domains of the Hemolytic Lectin CEL-III from the Marine Invertebrate Cucumaria echinata
• Conformation of the Primary Binding Loop Folded through an Intramolecular Interaction Contributes to the Strong Chymotrypsin Inhibitory Activity of the Chymotrypsin Inhibitor from Erythrina variegata Seeds.
• Molecular Cloning, Functional Expression, and Mutagenesis of cDNA Encoding a Cysteine Proteinase Inhibitor from Sunflower Seeds.
• Molecular Cloning and Functional Expression of cDNA Encoding the Cysteine Proteinase Inhibitor with Three Cystatin Domains from Sunflower Seeds.

もっと見る 閉じる

スポンサーリンク