Involvement of OS-2 MAP kinase in regulation of the large-subunit catalases CAT-1 and CAT-3 in Neurospora crassa

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概要

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• Neurospora crassa has four catalase genes—cat-1, cat-2, cat-3, and ctt-1/cat-4. cat-1 and cat-3 encode two fungal-specific large-subunit catalases CAT-1 and CAT-3 normally produced in conidia and growing hyphae, respectively. cat-2 encodes CAT-2 catalase-peroxidase normally produced in conidia. ctt-1 (or cat-4), of which expression was controlled by OS-2 MAP kinase (Noguchi et al., Fungal Genet. Biol. 44, 208–218), encodes a small-subunit catalase with unknown function. To clarify the contribution of OS-2 on the regulation of CAT-1, CAT-2, and CAT-3, we performed quantitative RT-PCR and in-gel catalase activity analyses. When the hyphae were treated with a fungicide (1 μg/ml fludioxonil) or subjected to an osmotic stress (1 M sorbitol), cat-1 was strongly upregulated and CAT-1 was reasonably induced in the wild-type strain. Interestingly, fludioxonil caused not only the CAT-1 induction but also a remarkable CAT-3 decrease in the wild-type hyphae, implying of an abnormal stimulation of asexual differentiation. These responses were not observed in an os-2 mutant hyphae, indicating an involvement of OS-2 in the cat-1 expression; however, os-2 was dispensable for the production of CAT-1 in conidia. In contrast, the expression of cat-2 was significantly induced by heat shock (45°C) and that of cat-3 was moderately stimulated by an oxidative stress (50 μg/ml methyl viologen) in both the wild-type strain and the os-2 mutant, and corresponding enzyme activities were detected after the treatments. Although basal levels of transcription of cat-1 and cat-3 in an os-2 mutant hyphae were a few-fold lower than in the wild-type hyphae, the os-2 mutant exhibited a considerably lower levels of CAT-3 activity than the wild-type strain. These findings suggest that OS-2 MAP kinase regulated the expression of cat-1 and cat-3 transcriptionally, and probably that of cat-3 posttranscriptionally, even though the presence of another regulatory system for each of these two genes is evident.

• 日本遺伝学会の論文
• 2007-08-25

著者

• FUJIMURA Makoto

  Faculty of Life Sciences, Toyo University

• ICHIISHI Akihiko

  Faculty of Life Sciences, Toyo University

• Fujimura Makoto

  Fac. Of Life Sciences Toyo Univ.

• Fujimura M

  Faculty Of Engineering Nagasaki University

• Fujimura M

  Univ. Toyo Oura‐gun Jpn

• Fujimura M

  Fac. Of Life Sciences Toyo Univ.

• Fujimura Makoto

  The Faculty Of Engineering Nagasaki University

• YAMASHITA Kazuhiro

  Faculty of Life Sciences, Toyo University

• SHIOZAWA Azusa

  Faculty of Life Sciences, Toyo University

• BANNO Shinpei

  Plant Function Research Center, Toyo University

• FUKUMORI Fumiyasu

  Faculty of Life Sciences, Toyo University

• KIMURA Makoto

  Plant & Microbial Metabolic Engineering Research Unit, Discovery Research Institute (DRI), RIKEN

• Kimura M

  Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University

• Fujimura Makoto

  Faculty Of Life Sciences Toyo University

• Ichiishi Akihiko

  Faculty Of Life Sciences Toyo University

• Kimura Makoto

  Plant & Microbial Metabolic Engineering Research Unit Discovery Research Institute (dri) Riken

• Kawabe Masato

  United Graduate School Of Agricultural Science Tokyo University Of Agriculture And Technology (tuat)

• Banno Shinpei

  Plant Function Research Center Toyo University

• Mimori Kouhei

  Laboratory For Remediation Research Plant Science Center Riken:department Of Engineering Toyo Univer

• Ichiishi Akihiko

  Fac. Of Life Sciences Toyo Univ.

• Yamashita Kazuhiro

  Faculty Of Engineering Science Osaka University

• Fukumori Fumiyasu

  Faculty Of Life Sciences Toyo University

• Shiozawa Azusa

  Faculty Of Life Sciences Toyo University

• Yamashita Kazuhiro

  Faculty Of Life Sciences Toyo University

• Kimura Makoto

  Digital Human Research Center Aist Japan

• KIMURA Makoto

  Plant & Microbial Metabolic Engineering Research Unit, Discovery Research Institute (DRI), RIKEN

• FUJIMURA Makoto

  Faculty of Life Science, University of Toyo

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