Biochemical Characterization of 60S Acidic Ribosomal P Proteins Associated with CK-II from Bamboo Shoots and Potent Inhibitors of Their Phosphorylation in Vitro
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概要
- 論文の詳細を見る
Three effective phosphate acceptors (35,15 and 13 kDa polypeptides) for casein kinase II (CK-II) in the Superdex CK-II fraction prepared from a 0.5 M NaCl extract of bamboo shoots were selectively purified by glycyrrhizin (GL)-affinity column chromatography (HPLC). These three proteins (p35,p15 and p13) were identified as 60S acidic ribosomal P proteins by determination of their partial N-terminal sequences CK-II was associated with p35 since the GL-affinity fraction was coprecipitated with an anti-serum against Drosophila CK-IIβ. Moreover, a derivative (oGA) of glycyrrhetinic acid (GA) and several polyphenol-containing anti-oxidative compounds [quercetin, epigallocatechin gallate (FGCG) and two isoflavones, i.e., 3', 4', 7-trihydroxyisoflavone (3', 4', 7-THI) and 8-chloro-3', 4', 5,7-tetrahydroxyisoflavone (8C-3', 4', 5,7-THI)] inhibited the CK-II-mediated phosphorylation of 60S acidic ribosomal P proteins in vitro. Quercetin was found to be the most effective compounds on CK-II activity since its ID_<50> was approx. 50 nM. These results suggest that (i) GL-affinity column chromatography is useful for the selective purification of 60S acidic ribosomal P proteins as a heterocomplex associated with CK-II from various cell sources; (ii) natural anti-oxidative compounds with polyphenols, but not GL and GA, act as potent CK-II suppressors; and (iii) CK-II mediates the regulation of the physiological functions of 60S acidic ribosomal P proteins in growing plant cells.
- 公益社団法人日本薬学会の論文
- 1999-07-15
著者
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MAEKAWA Toshiro
Laboratory of Genetical Biochemistry and Signal Biology, Graduate School of Medical Sciences, Kitasa
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OHTSUKI Kenzo
Laboratory of Genetical Biochemistry and Signal Biology, Graduate School of Medical Sciences, Kitasa
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KOMIYAMA Kanki
The Kitasato Institute
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Komiyama Kanki
The Kitasaro Institute And School Of Pharmaceutical Sciences The Kitasato University
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Ohtsuki Kenzo
Genetical Biochem. Kitasato Univ.
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Ohtsuki Kenzo
Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato
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FUNAYAMA Shinji
Department of Kampo Pharmaceutical Sciences, Nihon Pharmaceutical University
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Maekawa Toshiro
Laboratory Of Genetical Biochemistry Graduate School Of Medical Sciences Kitasato University
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Maekawa Toshiro
Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato
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KOSUGE Seiji
Laboratory of Genetical Biochemistry, School of Allied Health Sciences, Kitasato University
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SAKAMOTO Saki
Laboratory of Genetical Biochemistry, School of Allied Health Sciences, Kitasato University
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Kosuge Seiji
Laboratory Of Genetical Biochemistry Graduate School Of Medical Sciences Kitasato University
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Sakamoto Saki
Laboratory Of Genetical Biochemistry School Of Allied Health Sciences Kitasato University
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Ohtsuki K
Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato
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Funayama S
Dep. Of Kampo Pharmaceutical Sciences Nihon Pharmaceutical Univ.
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Funayama Shinji
Department Of Bioscience And Biotechnology
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Maekawa Toshiro
Laboratory For Digestive Diseases Center For Genomic Medicine Riken
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Ohtsuki Kenzo
Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato
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