Biochemical Characterization of Bovine Lactoferrin as a Glycyrrhizin-Binding Protein in Vitro
スポンサーリンク
概要
- 論文の詳細を見る
Lactoferring (LF) from bovine colostrum was biochemically characterized as a glycyrrhizin (GL)-binding protein (gbP) in vitro. It was found that (i) bovine LF (bLF) and a synthetic bovine lactoferricin (bLFcin, the N'-terminal region of bLF at the positions 17-41) had a high affinity to a GL-affinity column; (ii) approximaterly 1.8 moles of GL were bound to a molecule of bLF with a binding constant of approx. 1.20×10^4 M^<-1> at pH 6.8; and (iii) GL, but not glucurrhetinic acid (GA), induced a conformational change of bLF. In addition, the glucuronic acid moiety of the GL molecule was found to be responsible for binding to bLF, because (i) no binding of GA and two glucoses-GA (Glc-Glc-GA) to bLF was detected; and (ii) a synthetic fluorinated GL (GlcA-GlcF-GA) and mono-glucuronyl-GA (mono-GlcA-GA) were bound significantly to bLF. A similar binding of GL to human LF (hLF) was also observed under the same experimental conditions. Data provided here suggest that (i) bLF contains plural GL-binding sites; and (ii) the specific binding of GL to bLF may modulate the physiological activity of bLF in vivo.
- 公益社団法人日本薬学会の論文
- 2000-04-01
著者
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Tanaka Katsunao
Pharmacobioregulation Research Laboratory Taiho Pharmaceutical Co. Ltd.
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Tanaka Katsunao
大鵬薬品工業
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Tanigawa Kiyoshi
大鵬薬品工業
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Tanigawa Kiyoshi
Pharmacobioregulation Research Laboratory Taiho Pharmaceutical Co. Ltd.
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Ohtsuki K
Kitasato Univ. Sagamihara Jpn
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Ohtsuki Kenzo
Genetical Biochem. Kitasato Univ.
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Fujihara M
Kitasato Univ. Sagamihara Jpn
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Fujihara Michio
Laboratory Of Genetical Biochemistry School Of Allied Health Sciences Kitasato University
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大久保 研之
Department Of Clinical Chemistry And Laboratory Medicine Faculty Of Medicine Kyushu University
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Furuya T
Yamanouchi Pharmaceutical Co. Ltd. Ibaraki Jpn
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Furuya Toshio
Lead Discovery Laboratories Institute For Drug Discovery Research Yamanouchi Pharmaceutical Co. Ltd.
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Furuya Teisuke
Laboratory Of Genetical Biochemistry Kitasato University School Of Allied Health Sciences
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Ohtsuki K
Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato
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Tanigawa Kozo
Pharmacobioregulation Research Laboratory Taiho Pharmaceutical Co. Ltd.
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TANIGAWA KOZO
Laboratory of Chinical Chemistry, School of Allied Health Sciences, Kitasato University
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FUJIHARA Michio
Genetical Biochemistry, School of Allied Health Sciences, Kitasato University
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FUJIHARA Teisuke
Genetical Biochemistry School of Allied Health Sciences and Laboratory of Biochemistry
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SHIMOYAMA Yoshihito
Genetical Biochemistry School of Allied Health Sciences and Laboratory of Biochemistry
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MORISHIMA Naohiko
School of Nursing, Kitasato University
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Shimoyama Y
Kitasato Univ. Sagamihara Jpn
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Yanahira Shuichi
Technology And Research Institute Snow Brand Milk Products Co. Ltd.
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Shimoyama Yoshihito
Genetical Biochemistry And Signal Biology Graduate School Of Medical Science Kitasato University
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Furuya Teisuke
Genetical Biochemistry School Of Allied Health Sciences And Laboratory Of Biochemistry
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Tanigawa Kozo
Laboratory Of Chinical Chemistry School Of Allied Health Sciences Kitasato University
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Ohtsuki Kenzo
Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato
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Morishima N
School Of Nursing Kitasato University
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Morishima Naohiko
School Of Nursing Kitasato University
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