A transgene and its expression profile are stably transmitted to offspring in transgenic medaka generated by the particle gun method

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概要

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• A particle gun is used in a potential method for introducing foreign genes into fish. In this paper, we report on the stable transmission of a transgene and its expression profile of the F4 generation in the transgenic medaka (Oryzias latipes). We established four transgenic strains, which contained a green fluorescent protein (GFP) gene controlled by a medaka P-actin promoter, using a particle gun. One more transgenic strain was also generated by microinjection for comparison. In all five strains, the founder was discovered to be mosaic for the transgene. However, from the F1 to F4 generations, transgenes and their expression profiles were stably inherited in the Mendelian manner. The expression profile was common among the five strains regardless of the method for gene transfer: GFP fluorescence became detectable at an early neurula stage. In this stage, the fluorescence was observed ubiquitously in most tissues. As somite developed, GFP fluorescence became intense only in the skeletal muscle and lens but it decreased in other tissues. In adult fish, an intense fluorescence was restricted in the skeletal muscle and lens, while a considerably weak fluorescence was observed in the brain, gill, heart, kidney, spleen, and ovary. From these results, it was concluded that the transgene and its expression profile were stably transmitted to offspring, and thus the particle gun is an effective method for transgenesis in spite of its easiness.

• 社団法人日本動物学会の論文
• 2003-07-25

著者

• INOUE Koji

  Ocean Research Institute, The University of Tokyo

• Wakamatsu Yuko

  Laboratory of Freshwater Fish Stocks, Bioscience Center, Nagoya University

• Ozato Kenjiro

  Laboratory of Freshwater Fish Stocks, Bioscience Center, Nagoya University

• Inoue K

  Ocean Research Institute University Of Tokyo

• Osada Takashi

  Laboratory Of Freshwater Fish Stocks Bioscience Center Nagoya University

• Ishikawa Yuji

  National Inst. Radiological Sci. Chiba Jpn

• Ohtsuki K

  Kitasato Univ. Sagamihara Jpn

• Inoue Koji

  Ocean Research Institute University Of Tokyo

• Kinoshita Masato

  Division of Applied Bioscience, Graduate School of Kyoto University

• Yamauchi Masatake

  National Institute of Radiological Sciences

• Sasanuma Motoe

  National Institute of Radiological Sciences

• Kinoshita M

  Division Of Applied Biosciences Graduate School Of Agriculture Kyoto University

• Inoue K.

  Univ. Tokyo Tokyo Jpn

• Inoue K

  Semiconductor Group System-lsi Div. Mitsubishi Electric Co.

• Ohtsuki Kozo

  Department Of Food Sciences And Nutritional Health Kyoto Prefectural University

• Ozato K

  Laboratory Of Freshwater Fish Stocks Bioscience And Biotechnology Center Nagoya University

• Ozato Kenjiro

  Laboratory Of Freshwater Fish Stocks Bioscience And Biotechnology Center Nagoya University

• Wakamatsu Y

  Laboratory Of Freshwater Fish Stocks Bioscience And Biotechnology Center Nagoya University

• Wakamatsu Y

  Division Of Biological Science Graduate School Of Science Nagoya University

• Wakamatsu Yuko

  Laboratory Of Freshwater Fish Stocks Bioscience And Biotechnology Center Nagoya University

• Kanamori M

  Interdisciplinary Research Institute Of Environmental Sciences

• Inoue K

  Univ. Tokyo Tokyo Jpn

• Ito Kosei

  Graduate School Of Agricultural Science Tohoku University

• Kinoshita Masato

  Division Of Applied Bioscience Graduate School Of Agriculture Kyoto University

• Inoue Koji

  Ocean Research Institute The University Of Tokyo

• Kinoshita Masato

  Kyoto Univ. Kyoto

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