Estrogen inhibits development of yolk veins and causes blood clotting in transgenic medaka fish overexpressing estrogen receptor

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概要

• 論文の詳細を見る

• We established three transgenic medaka fish lines overexpressing the medaka estrogen receptor under the constitutive medaka beta-actin promoter. The transgenic embryos became hypersensitive to estrogens (17beta-estradiol and 17alpha-ethinylestradiol), and failed to develop yolk veins while blood clots formed in the blood island within 3 days after exposure to the estrogens. The embryos developed normally if exposed to estrogen after an early neurula stage, suggesting that the sensitive stage is before neurulation. The developmental defects were recovered by incubation with an anti-estrogen, tamoxifen. These results indicate that activation of estrogen receptor caused the estrogen-induced, developmental defects. Our results show that the transgenic embryos can be used to assay the blood clotting activity of estrogenic compounds in vivo.

• 社団法人日本動物学会の論文
• 2002-12-25

著者

• YAMASHITA Ichiro

  Center for Gene Science, Hiroshima University

• Kawamura Toshiyuki

  Center For Gene Science Hiroshima University

• Kinoshita Masato

  Division of Applied Bioscience, Graduate School of Kyoto University

• Hori-e Ryota

  Center for Gene Science, Hiroshima University

• Omura Seiichi

  Center for Gene Science, Hiroshima University

• Sakai Shuichi

  Center for Gene Science, Hiroshima University

• Kawahara Tetsuya

  Center for Gene Science, Hiroshima University

• Kawahara Tetsuya

  Center For Gene Science Hiroshima University

• Yamashita Ichiro

  Center For Gene Science Hiroshima University

• Kawamura T

  Department Of Molecular Biology Keio University School Of Medicine

• Yamashita I

  Center For Gene Science Hiroshima University

• Kinoshita M

  Division Of Biological Sciences Graduate School Of Science Hokkaido University

• Hori-e Ryota

  Center For Gene Science Hiroshima University

• Omura Seiichi

  Center For Gene Science Hiroshima University

• Kinoshita Masato

  Division Of Applied Bioscience Graduate School Of Agriculture Kyoto University

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