Cloning and Nucleotide Sequence of a Gene Conferring Ability to Grow at a Low Temperature on Saccharomyces cerevisiae Tryptophan Auxotrophs
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概要
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A gene conferring on yeast YNN140 the ability to grow at a low temperature was cloned and designated LTG3 (low temperature growth gene). Disruption of the LTG3 gene resulted in no growth at a low temeprature. It was shown that the effect of the presence of the gene in multicopy was dependent on the strain. DNA sequencing analysis of the LTG3 gene revealed on open reading frame which can encode 592 amino acids. The deduced amino acid sequence of the protein was homologous with that of some amino acid permeases. Since the addition of a large amount of _L-tryptophan to YPAD medium enabled the YNN140 strain to grow at a low temperature, it is possible that a limiting step for YNN140 to grow at a low temperature is to take up tryptophan from the medium. A part of the nucleotide sequence of the cloned DNA containing the LTG3 gene coincided with that of the SUP3 gene, indicating that the LTG3 gene exists on chromosome XV.
- 社団法人日本生物工学会の論文
- 1994-01-25
著者
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YAMASHITA Ichiro
Center for Gene Science, Hiroshima University
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Toh-e Akio
Laboratory Of Genetics Department Of Biology Faculty Of Science University Of Tokyo
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Nimi Osamu
Center For Gene Science Hiroshima University
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Yamashita Ichiro
Center For Gene Science Hiroshima University
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Yamashita I
Hiroshima Univ. Higashihiroshima Jpn
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Toh-e Akio
Laboratory Of Genetics Department Of Botany Faculty Of Science University Of Tokyo
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Toh-e Akio
Laboratory Of Frontier Science The Metropolitan Institute Of Medical Science
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KAWAMURA DAIZO
Hiroshima Prefectural Food Technological Research Center
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KAWAMURA Daizo
Hiroshima Food Research Institute
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