A cis-Acting Locus Needed for Stable Maintenance of the Yeast Plasmid pSB3(Microbiology & Fermentation Industry)

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概要

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• The Zygosaccharomyces rouxii plasmid pSB3 is one of the yeast plasmids resembling 2-μm DNA. The presence of a m-acting locus needed for stable maintenance of pSB3 has been expected by analogy with the functions encoded by other yeast plasmids such as 2-μm DNA and pSR1. We found such a locus and designated it STB-SB3. The STB-SB3 locus was delimited within 371 bp in a unique region of pSB3. This region is included in the plasmid region where no transcript was detected. No prominent feature of the nucleotide sequence was found in this region except for a direct repeat with 65% homology consisting of 27 bp and 29 bp. This is in clear contrast with the structure of the STB locus of 2-μm DNA which has five and a half tandem repeats consisting of a highly homologous 62 bp sequence. By analyzing the sites hypersensitive to nucleases using partially purified nuclei containing pSB3, the STB-SB3 locus was found not to be tightly folded into a nucleosome structure.

• 社団法人日本農芸化学会の論文
• 1990-09-23

著者

• Toh-e Akio

  Laboratory Of Genetics Department Of Biology Faculty Of Science University Of Tokyo

• Toh-e Akio

  Laboratory Of Frontier Science The Metropolitan Institute Of Medical Science

• IMURA Toshiaki

  Department of Fermentation Technology, Hiroshima Universily

• Imura Toshiaki

  Department Of Fermentation Technology Hiroshima University

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• A cis-Acting Locus Needed for Stable Maintenance of the Yeast Plasmid pSB3(Microbiology & Fermentation Industry)
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