Comparison of Thermal and Guanidine Hydrochloride Denaturation Behaviors of Glucoamylase from the STA1 Gene of Saccharomyces cerevisiae var. diastaticus

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概要

• 論文の詳細を見る

• To investigate the relationships between enzyme inactivation and conformational change, the effects of heat and guanidine hydrochloride (GnHCl) on the STA1 gene glucoamylase (STA1GA) of Saccharomyces cerevisiae var. diastaticus were examined by circular dichroism and fluorescence spectroscopies. A conformational change was observed in the thermal denaturation of STA1GA, while extensive enzyme inactivation occurred in GnHCl denaturation before noticeable conformational change.

• 社団法人日本農芸化学会の論文
• 1996-09-23

著者

• Ono Shin

  Department of Radiology, School of Veterinary Medicine, Azabu University

• YAMASHITA Ichiro

  Center for Gene Science, Hiroshima University

• YOSHIMURA Toshiaki

  Department of System Engineering of Materials and Life Science, Faculty of Engineering, Toyama Unive

• YAMAZAKI Isao

  Yayoi Kagaku Kogyo Co., Ltd.

• SHIMASAKI Choichiro

  Department of Material Systems Engineering and Life Science, Faculty of Engineering, Toyama Universi

• KATO Tetsuo

  Department of Applied Microbial Technology, Kumamoto Institute of Technology

• Ono S

  Toyama Univ. Toyama Jpn

• Shimasaki C

  Hokuriku Polytechnic College

• Shimasaki Choichiro

  Department Of System Engineering Of Materials And Life Science Faculty Of Engineering Toyama Univers

• Kato T

  Food Research Institute Aichi Prefectural Government

• MATSUMOTO Toshihiko

  Department of Applied Microbial Technology, Kumamoto Institute of Technology

• YAMAURA Izumi

  Department of Applied Microbial Technology, Kumamoto Institute of Technology

• Yamashita Ichiro

  Center For Gene Science Hiroshima University

• Matsumoto T

  Applied Biological Chemistry University Of Tokyo

• TANPA Sumie

  Department of Chemical and Biochemical Engineering, Toyama University

• Kato T

  Shizuoka Univ. Shizuoka Jpn

• Tanpa Sumie

  Department Of Chemical And Biochemical Engineering Toyama University

• Yamaura I

  Yayoi Kagaku Kogyo Co. Ltd.

• Yamaura Izumi

  Department Of Applied Microbial Technology The Kumamoto Institute Of Technology

• Yamazaki Isao

  Yayoi Kagaku Kogyo Co. Ltd. Fukuoka-machi

• Shimasaki Choichiro

  Department Of Chemical And Biochemical Engineering Faculty Of Engineering Toyama University

• Matsumoto Toshihiko

  Department Of Applied Microbial Technology The Kumamoto Institute Of Technology

• Yamaura I

  Department Of Applied Microbial Technology The Kumamoto Institute Of Technology

• Ono Shin

  Department Of Chemical And Biochemical Engineering Faculty Of Engineering Toyama University

• Yoshimura Toshiaki

  Department of Applied Chemistry, Faculty of Engineering, Osaka City University

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