Improved Purification and Spectroscopic Properties of Squash Glutamate Decarboxylase
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概要
- 論文の詳細を見る
Squash glutamate decarboxylase was purified by DEAE-Cellulose batchwise followed by Blue-Sepharose, Cellulofine GCL-2000, and Toyopearl HW-55F column chromatography. The purified glutamate decarboxylase had a high specific activity (95.0 u/mg). The absorption spectrum of glutamate decarboxylase had an absorption maximum at 420 nm in the range 300-500 nm. A pH change from 5.3 to 7.8 was accompanied by a decrease in absorbancy at 420 nm. One mole of glutamate decarboxylase contained 3.8 and 1.3 mol of pyridoxal 5'-phosphate at pH 5.8 and pH 7.8, respectively.
- 社団法人日本農芸化学会の論文
- 1996-05-23
著者
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YAMAZAKI Isao
Yayoi Kagaku Kogyo Co., Ltd.
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MATSUMOTO Toshihiko
Department of Applied Microbial Technology, Kumamoto Institute of Technology
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YAMAURA Izumi
Department of Applied Microbial Technology, Kumamoto Institute of Technology
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Matsumoto T
Applied Biological Chemistry University Of Tokyo
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FUNATSU Masaru
Department of Applied Microbial Technology, The Kumamoto Institute of Technology
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Yamaura I
Yayoi Kagaku Kogyo Co. Ltd.
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Yamaura Izumi
Department Of Applied Microbial Technology The Kumamoto Institute Of Technology
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Yamazaki Isao
Yayoi Kagaku Kogyo Co. Ltd. Fukuoka-machi
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Funatsu M
Department Of Applied Microbial Technology The Kumamoto Institute Of Technology
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Funatsu Masaru
Department Of Applied Microbial Technology The Kumamoto Institute Of Technology
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Matsumoto Toshihiko
Department Of Applied Microbial Technology The Kumamoto Institute Of Technology
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Yamaura I
Department Of Applied Microbial Technology The Kumamoto Institute Of Technology
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