Construction of Sandwich Enzyme Immunoassay for Rat Transthyretin Using Recombinant Antigen Approach, and Its Application
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概要
- 論文の詳細を見る
A sandwich enzyme immunoassay for rat transthyretin using a recombinant antigen approach is described. Rat transthyretin cDNA was cloned by PCR. Rat transthyretin fused with the IgG-binding ZZ domain of protein A was expressed in E. coli using the fusion vector pEZZ18. Antibody against the fusion protein was specific to rat transthyretin in plasma as shown by immunoblotting. Affinity-purified anti-rat transthyretin-ZZ was biotinylated and used for the sandwich enzyme immunoassay. In this assay, the measurable range was 1.2-50ng/ml and the coefficients of variation within and between the assay series (assay range : 2.5-20ng/ml) were 1.31±0.08% and 3.50±1.46%, respectively. Cross-reactivity was examined using bovine, human, and mouse serum. There was a cross-reaction only with mouse transthyretin. In an in vitro experiment, transthyretin secreted by rat hepatocytes could be measured by the sandwich enzyme immunoassay.
- 社団法人日本農芸化学会の論文
- 1993-03-23
著者
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Shirai Hiroki
Research Laboratory Of Applied Biochemistry Tanabe Seiyaku Company Ltd.
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Kishi Taiji
Laboratory Of Nutrition Chemistry Division Of Bioresource And Bioenvironmental Sciences Graduate Sch
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Kishi Taiji
九州大学 大学院
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Matsumoto T
Applied Biological Chemistry University Of Tokyo
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Matsumoto Takeshi
Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Company Ltd.
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Kishi Tetsuya
Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Company Ltd.
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Morita Motoyo
Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co., Ltd.
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Kishi Tetsuya
Laboratory Of Nutrition Chemistry Division Of Bioresource And Bioenvironmental Sciences Graduate Sch
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Kishi Tetsuya
Research Laboratory Of Applied Biochemistry Tanabe Seiyaku Co. Ltd.
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Morita M
Japan Women's Univ. Tokyo Jpn
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MATSUMOTO Takeshi
Research Center, Daicel Chemical Industries
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