Properties of Cuticular Phenoloxidase from Pupae of the Housefly, Musca domestica L.(Biological Chemistry)
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概要
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Cuticle-bound phenoloxidase was found in the pupae of the housefly (Musca domestica L.), and its properties were studied. The time course of dopachrome formation from dopa, and the effects of the amount of cuticles and dopa concentration on the formation of dopachrome from dopa by cuticle-catalyzed oxidation were studied. Cuticle-catalyzed oxidation was found to be an enzyme-catalyzed oxidation dependent on the cuticle-bound phenoloxidase. Km and V_<max> values for the oxidation of dopa by cuticle-bound phenoloxidase were 4.70 m_M and 1.68×10^<-2>U/mg cuticle, respectively. The optimal temperature of cuticle-bound phenoloxidase was approximately 45℃, and it was stable between 5 and 25℃. The optimal pH of cuticle-bound phenoloxidase was approximately 9.0, and it was stable from pH 7.0 to 9.5.
- 社団法人日本農芸化学会の論文
- 1991-01-23
著者
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YAMASAKI Nobuyuki
Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University
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WATANABE Keiichi
Laboratory of Biological Chemistry, Department of Biofunctional Chemistry, Faculty of Agriculture, K
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Yamasaki Nobuyuki
Laboratory Of Biochemistry And Laboratory Of Protein Chemistry And Engineering Faculty Of Agricultur
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FUNATSU Masaru
Department of Applied Microbial Technology, The Kumamoto Institute of Technology
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Hara Tatsuru
Laboratory Of Biological Chemistry Faculty Of Agriculture Saga University
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Funatsu M
Department Of Applied Microbial Technology The Kumamoto Institute Of Technology
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Funatsu Masaru
Department Of Applied Microbial Technology The Kumamoto Institute Of Technology
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TSUKAMOTO Takuji
Laboratory of Biological Chemistry, Faculty of Agriculture, Saga University
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Hara Toshio
Laboratory Of Biological Chemistry Faculty Of Agriculture Saga University
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Tsukamoto T
Laboratory Of Biological Chemistry Faculty Of Agriculture Saga University
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Yamasaki Nobuyuki
Laboratory Of Agricultural Process Engineering Faculty Of Agriculture Kyushu University
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Watanabe Keiichi
Laboratory Of Biological Chemistry Faculty Of Agriculture Saga University
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Watanabe Keiichi
Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University
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