Segmental Motion of Subsite C of Hen Egg-White Lysozyme : Fluorescence Depolarization Studies of Kyn62-Lysozyme as an Active Analogue
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概要
- 論文の詳細を見る
The dynamical properties of subsite C of hen egg-white lysozyme were investigated using Kyn62-lysozyme as an active analogue. Time-resolved fluorescence depolarization studies showed that the segmental motion of kynurenine which was important in subsite C was described with two components of which the rotational correlation times were φ_1=150ps and φ_2=1.4ns, respectively. Although these two segmental motions retained 90% of motional freedom, the slower motion was completely restricted and the degree of freedom was lost to 40% during the interaction with oligomers of N-acetyl-D-glucosamine.
- 社団法人日本農芸化学会の論文
- 1995-08-23
著者
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Yamashita Shoji
Faculty Of Agriculture Graduate School Of Kyushu Univ
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Nishimoto E
Kurume Univ. School Of Medicine Fukuoka Jpn
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Yamasaki Nobuyuki
Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University
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Yamasaki Nobuyuki
Laboratory Of Biochemistry And Laboratory Of Protein Chemistry And Engineering Faculty Of Agricultur
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Yamasaki N
Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University
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Yamashita S
Institute Of Biophysics Faculty Of Agriculture Graduate School Of Kyushu University
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Nishimoto Etsuko
Institute Of Biophysics Faculty Of Agriculture Graduate School Of Kyushu University
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Yamashita Shoji
Institute Of Biophysics Faculty Of Agriculture Graduate School Of Kyushu University
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Yamasaki Nobuyuki
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agr
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Yamasaki Nobuyuki
Laboratory Of Agricultural Process Engineering Faculty Of Agriculture Kyushu University
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