Arginine-55 in the β-Arm is Essential for the Activity of DNA-Binding Protein HU from Bacillus stearothermophilus

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概要

• 論文の詳細を見る

• DNA-binding protein HU (BstHU) from Bacillus stearothermophilus is a homodimeric protein which binds to DNA in a sequence-nonspecific manner. In order to identify the Arg residues essential for DNA binding, four Arg residues (Arg-53,Arg-55,Arg-58,and Arg-61) within the β-arm structure were replaced either by Gln, Lys, or Glu residues, and the resulting mutants were characterized with respect to their DNA-binding activity by a filter-binding analysis and surface plasmon resonance analysis. The results indicate that three Arg residues (Arg-55,Arg-58,and Arg-61) play a crucial role in DNA binding as positively charged recognition groups in the order of Arg-55>Arg-58>Arg-61 and that these are required to decrease the dissociation rate constant for BstHU-DNA interaction. In contrast, the Arg-53 residue was found to make no contribution to the binding activity of BstHU.

• 社団法人日本農芸化学会の論文
• 1999-12-23

著者

• Kawamura Shunsuke

  東北薬科大学 医療薬学

• Kawamura Shunsuke

  Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University

• YAMASAKI Nobuyuki

  Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University

• Kawamura Shunsuke

  Laboratorv Of Cell Biology. School Of Agriculture' Kvushu Tokai University

• KIMURA Makoto

  Laboratory of Structural Biology, Graduate School of Systems Life Sciences, Kyushu University

• Kawamura S

  Kyushu Tokai Univ. Kumamoto Jpn

• TANAKA Isao

  Division of Biological Science, Graduate School of Science, Hokkaido University

• Kimura M

  Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University

• Tanaka I

  Division Of Biological Science Graduate School Of Science Hokkaido University

• SAITOH Fumiyo

  Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University

• Kawamura S

  Department Of Physiology Keio University School Of Medicine

• Yamasaki Nobuyuki

  Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University

• Yamasaki Nobuyuki

  Laboratory Of Biochemistry And Laboratory Of Protein Chemistry And Engineering Faculty Of Agricultur

• Yamasaki N

  Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University

• Kawabe Masato

  United Graduate School Of Agricultural Science Tokyo University Of Agriculture And Technology (tuat)

• Saitoh Fumiyo

  Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University

• Tanaka I

  Fukui Prefecture Agricultural Experiment Station Fukui Jpn

• Kikukawa Shingo

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat

• Yamasaki Nobuyuki

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agr

• Kimura Makoto

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agr

• Yamasaki Nobuyuki

  Laboratory Of Agricultural Process Engineering Faculty Of Agriculture Kyushu University

• Kimura Makoto

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat

• Kimura Makoto

  Laboratory Of Biochemistry Department Of Agricultural Chemistry Faculty And Graduate School Of Agric

• KIMURA Makoto

  Laboratory for Remediation Research, Plant Science Center, RIKEN

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