The Contribution of Peripheral Stem-Loops to the Catalytic Activity of Archaeal RNase P RNA from Pyrococcus horikoshii OT3

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概要

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• We investigated the contribution of peripheral stem-loops to the catalytic activity of an archaeal RNase P RNA, PhopRNA, from Pyrococcus horikoshii OT3. PhopRNA mutants, in which the stem-loops were individually deleted, were prepared and characterized with respect to precursor tRNA (pre-tRNA) cleavage activity in the presence of five RNase P proteins. All the mutants retained the activity to some extent, indicating that they are moderately implicated in catalysis. Further characterization suggested that the stem-loops serve largely as binding sites for the proteins, and that their interactions are predominantly involved in stabilization of the active conformation of PhopRNA.

• 社団法人 日本農芸化学会の論文

著者

• Hara Tadashi

  Laboratory of Structural Biology, Graduate School of Systems Life Sciences, Kyushu University

• KIMURA Makoto

  Laboratory of Structural Biology, Graduate School of Systems Life Sciences, Kyushu University

• Nakashima Takashi

  Laboratory of Structural Biology, Graduate School of Systems Life Sciences, Kyushu University

• KAKUTA Yoshimitsu

  Laboratory of Structural Biology, Graduate School of Systems Life Sciences, Kyushu University

• Terada Atsushi

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat

• YAMAGUCHI Hiroki

  Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Graduate School, Faculty of

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