Expression of Nicotiana glutinosa Ribonucleases in Escherichia coli(Biochemistry & Molecular Biology)
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概要
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We previously isolated from Nicotiana glutinosa leaves three distinct cDNA clones, NGR1, NGR2, and NGR3, encoding a wound-inducible RNase NW, and putative RNases NGR2 and NGR3, respectively. In this study, we produced RNases NW and NGR3 in Escherichia coli and purified them to homogeneity. RNase NGR3 had non-absolute specificity toward polynucleotides, although RNase NW preferentially cleaved polyinosinic acid (Poly I). Both RNases NW and NGR3 were more active toward diribonucleoside monophosphates ApG, CpU, and GpU. Furthermore, kinetic parameters for RNase NW (K_m, 0.778 mM and k_<cat>, 1938min^<-1>) and RNase NGR3 (K_m, 0.548 mM and k_<cat>, 408 min^<-1>) were calculated using GpU as a substrate.
- 社団法人日本農芸化学会の論文
- 2002-04-23
著者
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KIMURA Makoto
Laboratory of Structural Biology, Graduate School of Systems Life Sciences, Kyushu University
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Kawano Shin
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat
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HINO Madoka
Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University
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Hino Madoka
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat
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Kawano Shin
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat
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Kimura Makoto
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agr
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Kimura Makoto
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat
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Kimura Makoto
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat
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Kimura Makoto
Laboratory Of Biochemistry Department Of Agricultural Chemistry Faculty And Graduate School Of Agric
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KIMURA Makoto
Laboratory for Remediation Research, Plant Science Center, RIKEN
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