Identification of Nucleotide Residues Essential for RNase P Activity from the Hyperthermophilic Archaeon Pyrococcus horikoshii OT3
スポンサーリンク
概要
- 論文の詳細を見る
Ribonuclease P (RNase P) is involved in the processing of the 5′ leader sequence of precursor tRNA (pre-tRNA). We have found that RNase P RNA (PhopRNA) and five proteins (PhoPop5, PhoRpp21, PhoRpp29, PhoRpp30, and PhoRpp38) reconstitute RNase P activity with enzymatic properties similar to those of the authentic ribozyme from the hyperthermophilic archaeon Pyrococcus horikoshii OT3. We report here that nucleotides A40, A41, and U44 at helix P4, and G269 and G270 located at L15/16 in PhopRNA, are, like the corresponding residues in Esherichia coli RNase P RNA (M1RNA), involved in hydrolysis by coordinating catalytic Mg2+ ions, and in the recognition of the acceptor end (CCA) of pre-tRNA by base-pairing, respectively. The information reported here strongly suggests that PhopRNA catalyzes the hydrolysis of pre-tRNA in approximately the same manner as eubacterial RNase P RNAs, even though it has no enzymatic activity in the absence of the proteins.
- 社団法人 日本農芸化学会の論文
著者
-
KIMURA Makoto
Laboratory of Structural Biology, Graduate School of Systems Life Sciences, Kyushu University
-
YOSHIDA Takeshi
Laboratory of Nutrition Chemistry, Department of Bioscience and Biotechnology, Faculty of Agricultur
-
Terada Atsushi
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat
関連論文
- Archaeal Homologs of Human RNase P Protein Pairs Pop5 with Rpp30 and Rpp21 with Rpp29 Work on Distinct Functional Domains of the RNA Subunit
- Crystal Structure of hyperthermophilic Archaeal Initiation Factor 5A : A Homologue of Eukaryotic Initiation Factor 5A (eIF-5A)
- The Structure of the Archaebacterial Ribosomal Protein S7 and Its Possible Interaction with 16S rRNA^1
- Missense Mutation in Abcg5 in SHRSP Rats Does Not Accelerate Intestinal Absorption of Plant Sterols: Comparison with Wistar Rats
- Characterization of Functional Domains of the Hemolytic Lectin CEL-III from the Marine Invertebrate Cucumaria echinata
- Expression in Cereal Plants of Genes That Inactivate Fusarium Mycotoxins(Biochemistry & Molecular Biology)
- Proteome Analysis of Wheat Lemma(Biochemistry & Molecular Biology)
- Cytotoxicity Induced by Erythrina variegata Serine Proteinase Inhibitors in Tumor Hematopoietic Stem Cell Lines
- Methanogenic archaeal communities developed in paddy fields in the Kojima Bay polder, estimated by denaturing gradient gel electrophoresis, real-time PCR and sequencing analyses(Soil Biology)
- Separate elements are required for ligand-dependent and -independent internalization of metastatic potentiator CXCR4
- Structural Changes in Ribonuclease P RNA in the Hyperthermophilic Archaeon Pyrococcus horikoshii OT3 Induced on Interaction with Proteins
- Crystal Structure and Functional Analysis of an Archaeal Chromatin Protein Alba from the Hyperthermophilic Archaeon Pyrococcus horikoshii OT3
- Origin and Properties of Humus in the Subsoil of Irrigated Rice Paddles : IV. Effect of Water Percolation on the Binding Type of Humus in a Submerged Plow Layer Soil
- Comparison of Humus Composition in the Subsoil of Japanese Paddy and Upland Fields
- Origin and Properties of Humus in the Subsoil of Irrigated Rice Paddies : III. Changes in Binding Type of Humus in Submerged Plow Layer Soil
- Origin and Properties of Humus in the Subsoil of Irrigated Rice Paddies : II. Effects of Subsoil Constituents and Drainage on the Accumulation of Organic Matter Leached from the Plow Layer Soil
- Origin and Properties of Humus in the Subsoil of Irrigated Rice Paddies : I. Leaching of Organic Matter from Plow Layer Soil and Accumulation in Subsoil
- Genomic Cloning of Ribonucleases in Nicotiana glutinosa Leaves, as Induced in Response to Wounding or to TMV-Infection, and Characterization of Their Promoters(Biochemistry & Molecular Biology)
- Contribution of Conserved Asn Residues to the Inhibitory Activities of Kunitz-Type Protease Inhibitors from Plants
- Protein Engineering of Novel Proteinase Inhibitors and Their Effects on the Growth of Spodoptera exigua Larvae(Biochemistry & Molecular Biology)
- Calcium Ions Stabilize a Protein Structure of Hemolytic Lectin CEL-III from Marine Invertebrate Cucumaria echinata
- Molecular Cloning and Functional Expression of cDNA Encoding the Cysteine Proteinase Inhibitor Sca from Sunflower Seeds
- Conformation of the Primary Binding Loop Folded through an Itramolecular Iteraction Contributes to the Strong Chymotrpsin Inhibitory Activity of the Chymotrypsin Inhibitor from Erythrina variegata Seeds
- Molecular Cloning, Functional Expression, and Mutagenesis of cDNA Encoding a Cysteine Proteinase Inhibitor from Sunflower Seeds
- Chymotrypsin Inhibitor from Erythrina variegata Seeds : Involvement of Amino Acid Residues within the Primary Binding Loop in Potent Inhibitory Activity toward Chymotrypsin
- Cloning, Expression, and Mutagenesis of Trypsin Inhibitor ETIb from Erythrina variegata Seeds
- The Tissue-Type Plasminogen Acitvator Inhibitor ETIa from Erythrina variegata: Structural Basis for the Inhibitory Activity by Cloning, Expression, and Mutagenesis of the cDNA Encoding ETIa
- Molecular Cloning of cDNA That Encodes Chymotrypsin Inhibitor ECI from Erythrina variegata Seeds and Its Expression in Escherichia coli
- Inhibitory Potency of Erythrina variegata Proteinase Inhibitors toward Serine Proteinases in the Blood Coagulation and Fibrinolytic Systems
- Purification, Characterization, and Sequencing of Two Cysteine Proteinase Inhibitors, Sca and Scb, from Sunflower (Helianthus annuus) Seeds
- Amino Acid Sequence of Chymotrypsin Inhibitor ECI from the Seeds of Erythrina variegata (LINN.) var. Orientalis
- Isolation and Primary Structure of Proteinase Inhibitors from Erythrina variegata (LINN.) var. Orientalis Seeds
- On the Interaction of Ribosomal Protein L5 with 5S rRNA(Biochemistry & Molecular Biology)
- Arginine-55 in the β-Arm is Essential for the Activity of DNA-Binding Protein HU from Bacillus stearothermophilus
- Contribution of a Salt Bridge to the Thermostability of DNA Binding Proten HU from Bacillus stearothermophilus Determined by Site-Directed Mutagenesis
- Characterization of the Archaeal Ribonuclease P Proteins from Pyrococcus horikoshii OT3
- Effect of elevated atmospheric CO_2 concentration on ammonia oxidizing bacteria communities inhabiting in rice roots(Soil Biology)
- A Wheat Xylanase Inhibitor Gene, Xip-I, but Not Taxi-I, Is Significantly Induced by Biotic and Abiotic Signals That Trigger Plant Defense
- New TAXI-type Xylanase Inhibitor Genes are Inducible by Pathogens and Wounding in Hexaploid Wheat
- Biotransformation of the Mycotoxin, Zearalenone, to a Non-estrogenic Compound by a Fungal Strain of Clonostachys sp.(Organic Chemistry)
- Structural Evidence for Endocrine Disruptor Bisphenol A Binding to Human Nuclear Receptor ERRγ
- Cloning of the Gene Encoding DNA Binding Protein HU from Bacillus stearothermophilus and Its Expression in Escherichia coli
- The Evolution of the Phenylacetic Acid Degradation Pathway in Bacteria
- Vertical changes in bacterial and archaeal communities with soil depth in Japanese paddy fields(Soil Biology)
- Molecular characterization of methane-oxidizing bacteria associated with rice straw decomposition in a rice field(Soil Biology)
- Primary Structure of 6.5k-Arginine/Glutamate-rich Polypeptide from the Seeds of Sponge Gourd (Luffa cylindrica)
- The Action of Rice Branching Enzyme I (BEI) on Starches
- Suppression of Ralstonia solanacearum in Soil Following Colonization by Other Strains of R. solanacearum
- Morphology, host range and phylogenetic diversity of Sphingomonas phages in the floodwater of a Japanese paddy field(Soil Biology)
- Abundance of bacteriophages of common heterotrophic bacteria in the floodwater of a Japanese paddy field(Soil Biology)
- Seasonal variations in the abundance of virus-like particles and bacteria in the floodwater of a Japanese paddy field(Soil Biology)
- Requirement for C-terminal Extension to the RNA Binding Domain for Efficient RNA Binding by Ribosomal Protein L2(Biochemistry & Molecular Biology)
- 5-8 Distribution of Photosynthetically Fixed C in Irrigated Rice : I. Contribution to Soil Dissolved Organic C and Microbial Biomass
- Effect of Rice Straw Application on CH_4 Emission from Paddy Fields : IV. Influence of Rice Straw Incorporated during the Previous Cropping Period
- Effect of Rice Straw Application on CH_4 Emission from Paddy Fields : III. Effect of Incorporation Site of Rice Straw on CH_4 Emission Rates and Their Variation among Shoots of a Rice Plant
- Methane Production and Its Fate in Paddy Fields : V. Fate of Methane Remaining in Paddy Soil at Harvesting Stage
- Activities of Soil Enzymes in Different Land-Use Systems in Middle Terrace Areas of Lampung Province, South Sumatra, Indonesia
- The Contribution of Peripheral Stem-Loops to the Catalytic Activity of Archaeal RNase P RNA from Pyrococcus horikoshii OT3
- Contribution of Gln9 and Phe8O to Substrate Binding in Ribonuclease MC1 from Bitter Gourd Seeds
- Amino Acids Conserved at the C-Terminal Half of the Ribonuclease T2 Family Contribute to Protein Stability of the Enzymes
- Expression and Mutational Analysis of Amino Acid Residues Involved in Catalytic Activity in a Ribonuclease MC1 from the Seeds of Bitter Gourd
- Molecular analysis of the ammonia oxidizing bacterial community in the surface soil layer of a Japanese paddy field(Soil Biology)
- Identification of Nucleotide Residues Essential for RNase P Activity from the Hyperthermophilic Archaeon Pyrococcus horikoshii OT3
- Observation of Microbial Colonization on the Surface of Rice Roots along with Their Development and Degradation
- Comparison of microbial community structure between soils suppressive and conducive to Fusarium-wilt of radish
- Epibiotic Microorganisms Associated with Microcrustaceans in Overlying Water of Philippine Paddy Fields
- Impact of Fumigation with Metam Sodium upon Soil Microbial Community Structure in Two Japanese Soils
- Seasonal Variation of Microcrustaceans and Microbial Flora on Their Surface in the Overlying Water of a Japanese Paddy Field
- Epibiotic Bacteria Associated with Microcrustaceans in the Overlying Water of Paddy Fields
- Isolation and Characterization of Bacteria Responsible for the Suppression of Fusarium oxysporum f. sp. raphani on the Host Rhizoplane
- Mechanisms of Suppression of Fusarium oxysporum f. sp. raphani in Soils So-Called Suppressive to Fusarium-Wilt of Radish
- Biochemical and Crystallographic Characterization of the Starch Branching Enzyme I (BEI) from Oryza sativa L
- Molecular Cloning of cDNAs Encoding Ribonuclease-related Proteins in Nicotiana glutinosa Leaves, as Induced in Response to Wounding or to TMV-infection(Biochemistry & Molecular Biology)
- Expression of Nicotiana glutinosa Ribonucleases in Escherichia coli(Biochemistry & Molecular Biology)
- Crystal Structures of the Nicotiana glutinosa Ribonuclease NT in Complex with Nucleoside Monophosphates
- The Action of Rice Branching Enzyme I (BEI) on Starches
- Biochemical and Crystallographic Characterization of the Starch Branching Enzyme I (BEI) from Oryza sativa L
- The C-Terminal Portion of an Archaeal Toxin, aRelE, Plays a Crucial Role in Protein Synthesis Inhibition
- Crystal Structure and Functional Analysis of an Archaeal Chromatin Protein Alba from the Hyperthermophilic Archaeon Pyrococcus horikoshii OT3
- Phenotypic Characterization of a Mutant of Burkholderia cepacia MRT11 Defective in Rhizosphere Competence
- Effects of Soil Temperature and Anaerobiosis on Degradation of Biodegradable Plastics in Soil and Their Degrading Microorganisms
- Missense Mutation in Abcg5 in SHRSP Rats Does Not Accelerate Intestinal Absorption of Plant Sterols : Comparison with Wistar Rats
- Characterization of ammonia oxidizing bacteria associated with weeds in a Japanese paddy field using amoA gene fragments(Soil Biology)
- 2P-019 マウス硫酸転移酵素SULT5A1のX線結晶構造解析(蛋白質・構造(2),第46回日本生物物理学会年会)
- The Contribution of Peripheral Stem-Loops to the Catalytic Activity of Archaeal RNase P RNA from Pyrococcus horikoshii OT3
- The Complete Amino Acid Sequences of the B-Chains of Abrin-a and Abrin-b, Toxic Proteins from the Seeds of Abrus precatorius
- N-linked glycan-dependent interaction of CD63 with CXCR4 at the Golgi apparatus induces downregulation of CXCR4
- Isolation and Molecular Characterization of Four Arginine/Glutamate Rich Polypeptides from the Seeds of Sponge Gourd (Luffa cylindrica)
- Identification of Nucleotide Residues Essential for RNase P Activity from the Hyperthermophilic Archaeon Pyrococcus horikoshii OT3