Isolation and Molecular Characterization of Four Arginine/Glutamate Rich Polypeptides from the Seeds of Sponge Gourd (Luffa cylindrica)

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概要

• 論文の詳細を見る

• Four arginine/glutamate rich polypeptides referred to as 5k-, 6.5k-, 12.5k-, and 14k-AGRPs were purified to homogeneity by gel filtration on Sephadex G-75 followed by CM-cellulose, butyl-Toyopearl 650M, and reverse-phase HPLC from the seed of sponge gourd (Luffa cylindrica). Tricine SDS-PAGE indicated that 5k-and 6.5k-AGRPs are single polypeptides, but 12.5k- and 14k-AGRPs consist of two polypeptide chains which are linked by disulfide bond(s). The N-terminal amino acid sequences of four AGRPs were analyzed by a gas-phase sequencer, and the result indicated that they are distinct molecules. Comparison of the sequences with those of proteins in the protein data base demonstrates that 5k- and 6.5k-AGRPs have a significant homology with a basic peptide from pumpkin seeds and with cocoa seed vicilin, respectively, and that 12.5k- and 14k-AGRPs are related to 2S seed storage proteins. Furthermore, it was assumed that the four AGRPs might occur in the protein bodies within cells of the seed.

• 社団法人日本農芸化学会の論文
• 1997-01-23

著者

• KIMURA Makoto

  Laboratory of Structural Biology, Graduate School of Systems Life Sciences, Kyushu University

• Kimura M

  Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University

• SAKAI Ritsu

  Laboratory of Biochemistry, Faculty of Agriculture, Kyusyu University

• Sakai Ritsu

  Laboratory Of Biochemistry Faculty Of Agriculture Kyusyu University

• Kawabe Masato

  United Graduate School Of Agricultural Science Tokyo University Of Agriculture And Technology (tuat)

• Funatsu G

  Kyushu Univ. Fukuoka Jpn

• Funatsu Gunki

  Laboratory Of Biochemistry And ** Laboratory Of Protein Chemistry & Engineering Faculty Of Agric

• Sasagawa Takahiro

  Laboratory Of Protein Chemistry And Engineering

• Ishihara Hisashi

  Laboratory of Protein Chemistry and Engineering

• Nishikawa Masateru

  Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University

• Kimura Makoto

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agr

• Nishikawa Masateru

  Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University

• Kimura Makoto

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat

• Kimura Makoto

  Laboratory Of Biochemistry Department Of Agricultural Chemistry Faculty And Graduate School Of Agric

• KIMURA Makoto

  Laboratory for Remediation Research, Plant Science Center, RIKEN

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