A Novel Transformation System for Pyricularia oryzae : Adhesion of Regenerating Fungal Protoplasts to Collagen-coated Dishes

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概要

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• A new method for transformation of fungi to give efficient selection was developed. Protoplasts of Pyricularia oryzae, treated by PEG-CaCl_2 with the blasticidin S (BS) deaminase gene (BSD) or the hygromycin phosphotransferase gene (hph), were transferred to liquid medium in collagen-coated dishes. They regenerated more efficiently in liquid than in agar, adhering to the collagen coating the dish. The colonies were continually kept under appropriate selective pressure by changing the medium. The BS resistant transformants of the isolate Hoku 1, well separated in the dish, were obtained at a frequency of 1000/μg vector. In isolate P2, the frequency of transformation was 190/μg vector with BSD and 4.5/μg vector with hph. The value was 20 and 6 times, respectively, higher than that obtained by the conventional method using agar plates. The selected colonies on collagen were easily picked up by forceps, which allowed efficient replica-plating.

• 社団法人日本農芸化学会の論文
• 1995-06-23

著者

• KOCHI Takamasa

  Tokyo University of Agriculture and Technology

• YAMAGUCHI Isamu

  Microbial Toxicology Laboratory, The Institute of Physical and Chemical Research (RIKEN)

• Kamakura T

  Graduate School Of Agriculture Tokyo University Of Agriculture And Technology

• Kamakura Takashi

  Riken The Institute Of Physical And Chemical Research

• Kimura M

  Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University

• Yamaguchi I

  Microbial Toxicology Laboratory Riken:(present Address)environmental Plant Research Group Plant Scie

• Yamaguchi Isamu

  Laboratory For Remediation Research Plant Science Center Riken

• KIMURA Makoto

  Microbial Toxicology Laboratory and Biodesign Research Group, RIKEN(The Institute of Physical and Ch

• Izawa Kiyoko

  Microbial Toxicology Laboratory, The Institute of Physical and Chemical Research (RlKEN)

• Yoneyama Katsuyoshi

  Microbial Toxicology Laboratory, The Institute of Physical and Chemical Research (RlKEN)

• Arie Tsutomu

  Microbial Toxicology Laboratory, The Institute of Physical and Chemical Research (RlKEN)

• Kamakura Takashi

  Biodesign Research Group, The Institute of Physical and Chemical Research (RlKEN)

• Yamaguchi Isamu

  Microbial Toxicology Laboratory Riken:(present Address)environmental Plant Research Group Plant Scie

• Arie Tsutomu

  Institute Of Symbiotic Science And Technology Tokyo University Of Agriculture And Technology (tuat)

• Arie Tsutomu

  Institute Of Symbiotic Science And Technology Tokyo University Of Agriculture And Technology

• Izawa Kiyoko

  Microbial Toxicology Laboratory The Institute Of Physical And Chemical Research (rlken)

• Kawabe Masato

  United Graduate School Of Agricultural Science Tokyo University Of Agriculture And Technology (tuat)

• Togashi Kana

  Graduate School Of Agriculture Tokyo University Of Agriculture And Technology

• Yoneyama K

  Shinshu Univ. Nagano Jpn

• Kimura Makoto

  Microbial Toxicology Laboratory And Biodesign Research Group Riken(the Institute Of Physical And Che

• YAMAGUCHI Isamu

  Microbial Toxicology Laboratory and Biodesign Research Group, RIKEN(The Institute of Physical and Chemical Research)

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