Production of Biodegradable Polyester by a Transgenic Tobacco

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概要

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• The acetoacetyl-CoA reductase gene (phbB) of Ralstonia eutropha and the poly [(R)-(-)-3-hydroxyalkanoate] synthase gene (phaC_AC) of Aeromonas caviae were introduced into tobacco plant by Agrobacterium mediated transformation method. The resulting transgenic tobacco expressed both introduced genes and the expression of these genes was confirmed by enzymatic analysis and western blotting. GC-MS analysis of the chloroform extract of tobacco leaves indicated that the transgenic plant produced biodegradable polyester, poly-[(R)-(-)-3-hydroxybutyrate]. GPC analysis indicated that the numberaverage molecular weights (Mn) and polydispersity (Mw^^^-/Mn^^^-) were 32,000 and 1.90,respectively.

• 社団法人日本農芸化学会の論文
• 1999-05-23

著者

• Kawano H

  Extremobiosphere Research Center (xbr) Japan Agency For Marine-earth Science And Technology (jamstec

• YAMAGUCHI Isamu

  The institute of Physical and Chemical Research

• ARAI Yuko

  Microbial Toxicology Laboratory, RIKEN Institute

• DOI Yoshiharu

  RIKEN Institute

• Nakashita Hideo

  Environmental Molecular Biology Laboratory Discovery Research Institute Riken

• USAMI Ron

  Toyo University

• HORIKOSHI Kohki

  Toyo University

• NAKASHITA Hideo

  The Institute of Physical and Chemical Research (RIKEN)

• ARAI Yuko

  Toyo University

• YOSHIOKA Keiko

  The Institute of Physical and Chemical Research (RIKEN)

• FUKUI Toshiaki

  The Institute of Physical and Chemical Research (RIKEN)

• DOI Yoshiharu

  The Institute of Physical and Chemical Research (RIKEN)

• Yamaguchi I

  Microbial Toxicology Laboratory Riken:(present Address)environmental Plant Research Group Plant Scie

• Yamaguchi Isamu

  Laboratory For Remediation Research Plant Science Center Riken

• Yamaguchi Isomaro

  Department Of Applied Biological Chemistry The University Of Tokyo

• Yamaguchi I

  Department Of Applied Biological Chemistry University Of Tokyo

• Usami R

  Department Of Applied Chemistry Faculty Of Engineering Toyo University

• Usami R

  Toyo Univ. Saitama Jpn

• Arai Y

  Microbial Toxicology Laboratory Riken Institute:graduate School Of Science And Engineering Saitama U

• Horikoshi K

  Extremobiosphere Res. Center (xbr) Japan Agency For Marine-earth Sci. And Technol. (jamstec)

• Doi Yoshiharu

  Polymer Chemistry Laboratory Riken Institute

• Usami Ron

  Graduate School Of Interdisciplinary New Science Toyo University

• Yoneyama K

  Shinshu Univ. Nagano Jpn

• Yamaguchi Isomaro

  Dept. Appl. Biol. Chem. Tokyo Univ.

• Yosioka Keiko

  The Institute of Physical and Chemical Research (RIKEN)

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