Physiological Properties of a Neutralo-sensitive Mutant Derived from Facultative Alkaliphilic Bacillus sp. C-125

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概要

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• A neutralo-sensitive mutant (M-12) was isolated from the facultative alkaliphilic Bacillus sp. C-125. This mutant strain was able to grow to the same extent as did the parent strain above pH 8,but did not grow below pH 7.5. The same extent of oxygen uptake was shown by the cells of the parent and mutant strains at pH 10.3. On the other hand, the oxygen uptake rate was about one-fifth of that of the parent strain at pH 7. NADH-dependent oxygen uptake by everted vesicles of the mutant was lower than that of the parent strain at pH 7-7.5,while the rate at pH 8-9 was almost identical in both strains. The activity at pH 7 of cytochrome c oxidase of right-side-out membrane vesicles of the mutant strain was lower than that of the farent strain at pH 7,while both samples had almost the same enzymatic activity at pH 8.5. These results suggest that poor respiratory activities of the mutant strain at pH 7 are the reason why this mutant strain was unable to grow at neutral pH.

• 社団法人日本農芸化学会の論文
• 1998-04-23

著者

• Kudo Toshiaki

  Environmental Molecular Biology Lab. :graduate School Of Integrated Science Yokohama City University

• Kudo Toshiaki

  Laboratory Of Microbiology The Institute Of Physical And Chemical Research(riken)

• KUDO Toshiaki

  Microbiology Laboratory, RIKEN

• Kawano H

  Extremobiosphere Research Center (xbr) Japan Agency For Marine-earth Science And Technology (jamstec

• Takio K

  Enviromental Molecular Biology Lab. Riken

• Kudo T

  Riken Saitama Jpn

• Kudo Toshiaki

  International Graduate School Of Arts And Sci. Yokohama City Univ.

• Kudo Toshiaki

  Yokohama City Univ. Kanagawa Jpn

• Kitada M

  Graduate School Of Integrated Science Yokohama City University

• Kitada Makio

  Microbiology Laboratory The Institute Of Physical And Chemical Research (riken)

• MOROTOMI Shinya

  Microbiology Laboratory, The Institute of Physical and Chemical Research (Riken)

• USAMI Ron

  Bioengineering Laboratory, Department of Applied Chemistry, Toyo University

• HORIKOSHI Koki

  Bioengineering Laboratory, Department of Applied Chemistry, Toyo University

• Kudo Toshiaki

  Environmental Molecular Biology Laboratory Riken

• Kudo Toshiaki

  Microbial Toxicology Laboratory The Institute Of Physical And Chemical Research

• Morotomi Shinya

  Microbiology Laboratory The Institute Of Physical And Chemical Research (riken):bioengineering Labor

• Usami R

  Department Of Applied Chemistry Faculty Of Engineering Toyo University

• Usami R

  Toyo Univ. Saitama Jpn

• Horikoshi K

  Extremobiosphere Res. Center (xbr) Japan Agency For Marine-earth Sci. And Technol. (jamstec)

• Usami Ron

  Graduate School Of Interdisciplinary New Science Toyo University

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