Characterization of the NADH-Linked Acetylacetoin Reductase/2, 3-Butanediol Dehydrogenase Gene from Bacillus cereus YUF-4

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概要

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• A 1.4-kbp DNA fragment, including the NADH-linked acetylacetoin reductase/2, 3-butanediol dehydrogenase(AACRII/BDH)gene from the chromosomal DNA of Bacillus cereus YUF-4, was cloned in Escherichia coli DH5α after its insertion into pUC119, and the resulting plasmid was named pAACRII119. The AACRII/BDH gene had an open reading frame consisting of 1047 bp encoding 349 amino acids. The enzyme exhibited not only AACR activity, but also BDH activity. However, the gene was not located in a 2, 3-butanediol(BD)operon, as is the case in the BDH gene of Klebsiella pneumoniae and that of K.terrigena. In addition, there was no BD-cycle-related enzyme gene in the region surrounding the AACRII/BDH gene. The AACR and BDH activities in E.coli DH5α/pAACRII119 were 200-fold higher than those in the original B.cereus YUF-4. The characteristics of the AACRII/BDH from E.coli DH 5α/pAACRII119 are similar to those of the AACRII/BDH from B.cereus YUF-4. The AACRII/BDH was considered to belong to the NAD(P)- and zinc-dependent long-chain alcohol dehydrogenase(group I ADH)family on the basis of the following distinctive characteristics: it possessed 14 strictly conserved residues of microbial group I ADH and consisted of about 350 amino acids. The enzymatic and genetic characteristics of AACRII/BDH were completely different from those of BDHs belonging to the short-chain dehydrogenase/reductase family. These findings indicated that the AACRII/BDH could be considered a new type of BDH.

• 社団法人日本生物工学会の論文
• 2001-06-25

著者

• Ui Sadaharu

  Division of Biotechnology Yamanashi Univ.

• Ohkuma Moriya

  Environmental Molecular Biology Laboratory Riken

• Ohkuma M

  Enviromental Molecular Biology Lab. Riken

• Ohkuma Moriya

  Department Of Agricultural Chemistry The University Of Tokyo

• Ui Sadaharu

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University

• Ui Sadaharu

  Division Of Biotechnology Interdisciplinary Graduate School Of Medicine And Engineering University O

• Kudo Toshiaki

  Environmental Molecular Biology Lab. :graduate School Of Integrated Science Yokohama City University

• Kudo Toshiaki

  Laboratory Of Microbiology The Institute Of Physical And Chemical Research(riken)

• Ohkuma Moriya

  Bio-recycle Project Japan Science And Technology Agency:presto Japan Science And Technology Agency:e

• Ui Sadaharu

  Department Of Applied Chemistry & Biotechnology Faculty Of Engineering Yamanashi University

• OHTSUKI Takashi

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University

• MIMURA Akio

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University

• OHKUMA Moriya

  Laboratory of Microbiology, The Institute of Physical and Chemical Research (RIKEN)

• HOSAKA TAKESHI

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University

• Takio Koji

  Riken

• Hosaka T

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University

• Ohtsuki Takashi

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering University Of Yamanashi

• Takio K

  Enviromental Molecular Biology Lab. Riken

• Kudo T

  Inst. Physical And Chemical Res.(riken) Saitama Jpn

• Kudo T

  Riken (inst. Physical And Chemical Res.) Wako Jpn

• Kudo T

  Environmental Molecular Biology Laboratory Riken

• Ohkuma Moriya

  Bio-recycle Project Japan Science And Technology Agency

• Kudo T

  Riken Saitama Jpn

• Kudo Toshiaki

  International Graduate School Of Arts And Sci. Yokohama City Univ.

• Kudo Toshiaki

  Yokohama City Univ. Kanagawa Jpn

• Mimura A

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering University Of Yamanashi

• Mimura A

  Hitachi Ltd. Ibaraki Jpn

• Hosaka Takeshi

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University

• Mimura A

  Univ. Of Yamanashi Yamanashi Jpn

• Mimura Akiko

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering University Of Yamanashi

• Kudo Toshiaki

  Environmental Molecular Biology Laboratory Riken

• Kudo Toshiaki

  Laboratory Of Microbiology The Institute Of Physical And Chemical Research

• Ohtsuki Takashi

  Department Of Applied Biology Faculty Of Textile Science And Technology Shinsyu University

• Takio K

  Biomolecular Characterization Division Riken

• Mimura Akio

  Department Of Applied Chemistry & Biotechnology Faculty Of Engineering Yamanashi University

• Ui S

  Division Of Biotechnology Interdisciplinary Graduate School Of Medicine And Engineering University O

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